过刊浏览

大鼠脑实质内远位触液神经元中5--HT_(1A)受体的分布及其在神经病理性痛中的表达

蒋文旭, 张励才

徐州医学院江苏省麻醉学重点实验室.江苏,徐州 221002

摘要

该文旨在探讨大鼠脑实质内远位触液神经元5--HT_(1A)受体的分布及其在神经病理性疼痛中的作用。慢性结扎损伤坐骨神经建立大鼠神经病理性疼痛模型，分别以缩足潜伏期(paw withdrawal latency, PWL)和缩足阈值 (paw withdrawal threshold, PWT)对大鼠热痛敏和机械触诱发痛反应进行评分，以可靠CB--HRP (cholera toxin subunit B with horseradish peroxidase)法追踪标记脑实质内远位触液神经元，用CB--HRP/5--HT_(1A)受体免疫组织化学双重标记技术定位、鉴别5--HT_(1A)受体在远位触液神经元的表达，并计数分析5--HT_(1A)受体变化与痛行为表现之间的关系。结果表明，在神经病理性疼痛d1、d3、d7、d14，大鼠的PWL分别为19.37±2.74、12.04±1.77、8.74±1.15、12.31±1.94， PWT分别为18.58±3.62、13.05±1.81、6.66±1.43、11.55±2.01。CB--HRP标记细胞出现的位置和数量恒定。每只动物CB--HRP/5--HT_(1A)受体双重标记的细胞数量分别为276.14±36、161.72±28.41、108.64±6.81、139.76±44.64，分别占该动物CB--HRP标记细胞总数的95%，60%，40%和55%。与对照组相比，PWL和PWT及CB--HRP/5--HT_(1A)受体双标细胞的均有显著性差异({sl P}<0.01)。结果提示，脑实质的特定部位恒定存在远位触液神经元，该类神经元大多含有5--HT_(1A)受体，该受体的表达与神经病理性疼痛行为学表现之间呈负相关关系。

关键词： 触液神经元; 5-HT_(1A)受体; 神经病理性痛; 大鼠

Distribution and effects of 5--HT_(1A) receptors in distal cerebral spinal fluid--contacting neurons in rat brain parenchyma in neuropathic pain

Jiang Wenxu, Zhang Licai

The Affiliated Nanjing Children's Hospital of Nanjing Medical University.Nanjing 210008,Jiangsu

Abstract

The present study aimed to explore the effects of 5-HT_(1A) receptors in the distal cerebral spinal fluid-contacting neurons(CSF-CNs) in rat brain parenchyma in neuropathic pain. The model of neuropathic pain with chronic constriction injury (CCI) of thesciatic nerve was made in Sprague-Dawley rats. The behavioral studies of animal were scored and the paw withdrawal latency (PWL)and paw withdrawal threshold (PWT) were measured. The distribution and expression of 5-HT_(1A) receptors were observed in the distalCSF-CNs in brain parenchyma with double labeling of cholera toxin subunit B with horseradish peroxidase (CB-HRP) and 5-HT_(1A) receptors with immunhistochemistry. The relationship between 5-HT_(1A) receptors in distal CSF-CNs and neuropathic pain wasanalyzed. The results were as follows. On days 1, 3, 7, 14 of neuropathic pain, the PWL was 19.37±2.74, 12.04±1.77, 8.74±1.15 and12.31±1.94, respectively; the PWT was 18.58±3.62, 13.05±1.81, 6.66±1.43 and 11.55±2.01, respectively. CB-HRP-labeled neuronsof two clusters were always found in definite region but not in other area in brain parenchyma. The number of neurons double-labeledwith CB-HRP/5-HT_(1A) receptors in each group was 276.14±36.00, 161.72±28.41,108.64±6.81, and 139.76±44.64, which was about95%, 60%, 40% and 55% of all CB-HRP-iabeled neurons in the four courses of neuropathic pain, respectively. It is suggested that thedistal CSF-CNs are always located in a special region in rat brain parenchyma and most of them have 5-HT_(1A) receptors. A negativecorrelation is found between the expression of 5-HT_(1A) receptors and neuropathic pain.

Key words: cerebral spinal fluid-contacting neuron;5-HT_(1A) receptor;neuropathic pain;Rat

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

蒋文旭, 张励才. 大鼠脑实质内远位触液神经元中5--HT_(1A)受体的分布及其在神经病理性痛中的表达[J]. 生理学报 2008; 60 (2): 243-248.

Jiang Wenxu, Zhang Licai. Distribution and effects of 5--HT_(1A) receptors in distal cerebral spinal fluid--contacting neurons in rat brain parenchyma in neuropathic pain. Acta Physiol Sin 2008; 60 (2): 243-248 (in Chinese with English abstract).