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中枢神经系统去甲肾上腺素分泌的实时检测

黄维, 黄宏平, 穆玉, 张磊, 金木, 吕靖, 古靓丽, 修云, 张勃, 郭宁, 刘涛, 孙蕾, 宋美英, 张曦, 阮怀珍, 周专

第三军医大学基础部.重庆 400038；北京大学分子医学研究所.北京 100871

摘要

为了探讨与中枢神经系统单胺类递质分泌失调有关疾病的中枢机制,人们对单胺类递质分泌动力学的研究越来越有兴趣。去甲肾上腺素是中枢神经系统重要的递质和调质,该文介绍了作者实验事最近发展的实时检测中枢神经系统去甲肾上腺素分泌的一些技术疗法,并比较了电化学微碳纤电极(carbon fiber electrode,CFE)测量与电生理、荧光显微测量技术优缺点,阐述了CFE技术在神经科学研究中的一个基本应用。

关键词： 中枢神经系统; 去甲肾上腺素; 分泌; 实时检测

Real--time measurement of noradrenaline release in central nervous system

Huang Wei, Huang Hongping, Mu Yu, Zhang Lei, Jin Mu, Lu Jing, Gu Liangli, Xiu Yun, Zhang Bo, Guo Ning, Liu Tao, Sun Lei, Song Meiying, Zhang Xi, Ruan Huaizhen, Zhou Zhuan

College of Basic Medicine, The Third Military Medical University.Chongqing 400038；China

Abstract

In order to investigate the central nervous mechanism and the diseases involved in catecholamine transmitter secretion,the dynamics of catecholamine release is studied in single cell,brain slice or in vivo.Noradrenaline is an important neurotransmitter and modulator in the central nervous system(CNS)and the peripheral nervous system (PNS).In the present paper,we first compared three real-time methods used to measure noradrenaline secretion in single cells(membrane capacitance,amperometry and confocal fluorescence microscopy imaging)．Compared to the electrophysiological method and fluorescence microscopy，the basic usage of the carbon fiber electrode(CFE)in neuroscience research was presented as an example．Then，we presented a primary description of ion channels，including voltage-gated Na~(+), K~(+) and Ca~(2+) channels in locus coeruleus(LC)neurons in rat brain sfices．Finally，we presented example recordings of combined patch-clamp and amperometry measurements in LC neurons，indicating Ca~(2+)-dependent quantal noradrenaline release following Ca~(2+) influx through Ca~(2+) channels．

Key words: Central nervous system;Noradrenaline;Secretion;real-time measurement

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引用本文：

黄维, 黄宏平, 穆玉, 张磊, 金木, 吕靖, 古靓丽, 修云, 张勃, 郭宁, 刘涛, 孙蕾, 宋美英, 张曦, 阮怀珍, 周专. 中枢神经系统去甲肾上腺素分泌的实时检测[J]. 生理学报 2007; 59 (6): 865-870.

Huang Wei, Huang Hongping, Mu Yu, Zhang Lei, Jin Mu, Lu Jing, Gu Liangli, Xiu Yun, Zhang Bo, Guo Ning, Liu Tao, Sun Lei, Song Meiying, Zhang Xi, Ruan Huaizhen, Zhou Zhuan. Real--time measurement of noradrenaline release in central nervous system. Acta Physiol Sin 2007; 59 (6): 865-870 (in Chinese with English abstract).