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低浓度神经生长因子存在下GPI--1046刺激鸡胚神经节突起生长

阙海萍, 李欣, 李松, 刘少君

军事医学科学院基础医学研究所神经生物学研究室.北京 100850；军事医学科学院毒物药物研究所.北京 100850

摘要

对GPI--1046是否具有神经营养作用目前有两种不同的认识。Steiner等认为GPI--1046能促进体外培养的感觉神经节神经元突起生长。但Harper等却没能证明GPI--1046有这样的作用。由于GPI--1046在临床上具有重要应用价值和前景,作者重新评价了GPI--1046对体外培养鸡胚神经节的神经营养作用,发现在低浓度神经生长因子(nerve growth factor,NGF)存在下,GPI--1046能明显促进鸡背根神经节神经突起的生长。

关键词： GPI-1046; 神经生长因子; 免疫亲和素; 神经突起; 鸡胚背根节

GPI--1046 stimulates chicken dorsal root ganglion neurite outgrowth in the presence of nerve growth factor at low concentration {sl in vitro}

Que Haiping, Li Xin, Li Song, Liu Shaojun

Department of Neurobiology,Institute of Basic Medical Sciences,Academy of Military Medical Sciences.Beijing 100850；China

Abstract

The purpose of this investigation is to re-evaluate the neurotrophic effect of GPI-1046 on neurite outgrowth in vitro. GPI-1046 was synthesized and identified with mass spectrometry, nuclear magnetic resonance and elemental analysis. Chicken dorsal root ganglions (DRG) were removed and divided into three groups: (1)The DRGs were cultured in DMEM containing different concentra- tions of GPI-1046;(2) The DRGs were cultured in DMEM containing nerve growth factor (NGF) alone at 0.8 and 8 ng/mL,respectively; (3)The DRGs were in DMEM containing both different concentration of GPI-1046 and NGF at 0.8 ng/mL. The results showed that GPI-1046 alone could not stimulate chicken DRG neurite outgrowth; however, GPI-1046 might magnify DRG neurite outgrowth only in the presence of low concentration of NGF in the culture medium.

Key words: GPI-1046;Nerve growth factor;immunophilin;neurite outgrowth;chicken dorsal root ganglia

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引用本文：

阙海萍, 李欣, 李松, 刘少君. 低浓度神经生长因子存在下GPI--1046刺激鸡胚神经节突起生长[J]. 生理学报 2007; 59 (6): 791-795.

Que Haiping, Li Xin, Li Song, Liu Shaojun. GPI--1046 stimulates chicken dorsal root ganglion neurite outgrowth in the presence of nerve growth factor at low concentration {sl in vitro}. Acta Physiol Sin 2007; 59 (6): 791-795 (in Chinese with English abstract).