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小鼠海马CAl区高频刺激诱发的突触可塑性分析

刘喜娟, 黄汾生, 黄辰, 杨章民, 冯新正

西南交通大学医学院遗传学与分子生物学系/环境与疾病相关基因教育部重点实验室.陕西,西安 710061；西南交通大学医学院生理学与病理生理学系.陕西,西安 710061；陕西师范大学生命科学学院.陕西,西安 710062

摘要

通过细胞外记录方法记录场兴奋性突触后电位(field excitatory postsynaptic potential, fEPSP)的变化是研究突触可塑性，诸如长时程增强(long--term potentiation, LTP)和双脉冲可塑性(paired--pulse plasticity, PPP)的最常见的方法之一。测量fEPSP波形的起始斜率、起始面积、峰值及总面积等的变化经常作为判断突触可塑性增强或减弱的判断标准。在相同记录结果中测量fEPSP波形不同部位通常会有不同的结果，因此可能得出不同的结论，但这往往会被一些研究者所忽略。该文通过测量fEPSP波形的起始斜率、起始面积、峰值、总面积及时间参数等分析和比较高频刺激(high--frequency stimulation, HFS)诱发的突触可塑性，包括LTP和PPP的变化。结果显示LTP过程中AMPA受体动力学变化加快，且在同一记录中，fEPSP波形不同部位的测量分析可以产生较大幅度的LTP和PPP差异，有些差异甚至可以导致相反的结论。

关键词： 分析; 长时程增强; 双脉冲可塑性; 海马; 小鼠

Analysis of high--frequency stimulation--evoked synaptic plasticity in mouse hippocampal CA1 region

Liu Xijuan, Huang Fensheng, Huang Chen, Yang Zhangmin, Feng Xinzheng

Department of Genetics and Molecular Biology, Medical School of Xi'an Jiaotong University/Key Laboratory of Environment and Genes Related to Diseases, Ministry of Education.Xi'an 710061,Shaanxi；China；College of Life Science, Shaanxi Normal University.Xi'an 710062,Shaanxi

Abstract

Extracellular recordings of field excitatory postsynaptic potential (fEPSP) is one of the most common ways for studies of synaptic plasticity, such as long-term potentiation (LTP) and paired-pulse plasticity (PPP). The measurement of the changes in the different components of fEPSP waveform, such as initial slope, initial area, peak amplitude and whole area, were commonly used as criteria for the judgment of potentiation or depression of synaptic plasticity. However, the differences of the conclusions drawn from measuring different components of fEPSP waveform at the same recording have still been largely ignored. Here we compared high-frequency stimulation (HFS)-evoked synaptic plasticity, both LTP and PPP, by measuring different components of fEPSP waveform, including initial slope, initial area, peak amplitude, the whole area and the time courses. The results not only indicated the acceleration of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor kinetics underlies LTP in hippocampal CA1 region of mice, but also showed that different measurements of fEPSP waveform at the same recordings resulted in different magnitude of LTP and different forms of PPP in hippocampal CA1 region of mice, and some of them have drawn apparently contradictory conclusions.

Key words: analysis;Long-term potentiation;paired-pulse plasticity;Hippocampus;Mice

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引用本文：

刘喜娟, 黄汾生, 黄辰, 杨章民, 冯新正. 小鼠海马CAl区高频刺激诱发的突触可塑性分析[J]. 生理学报 2008; 60 (2): 284-291.

Liu Xijuan, Huang Fensheng, Huang Chen, Yang Zhangmin, Feng Xinzheng. Analysis of high--frequency stimulation--evoked synaptic plasticity in mouse hippocampal CA1 region. Acta Physiol Sin 2008; 60 (2): 284-291 (in Chinese with English abstract).