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血小板活化因子对大鼠黄体细胞孕酮分泌及血管内皮生长因子表达的作用

郑慧丽, 温海霞, 刘国艺, 倪江

哈尔滨医科大学生理学教研室.黑龙江,哈尔滨 150086

摘要

该文旨在研究血小板活化因子(platelet--activating factor, PAF)对大鼠黄体细胞孕酮分泌及血管内皮生长因子(vascular endothelial growth factor, VEGF) mRNA表达的作用。将未成年(25---28 d) Spague--Dawley雌性大鼠颈部皮下注射孕马血清促性腺激素(pregnant mare serum gonadotrophin, PMSG)和人绒毛膜促性腺激素(human chorionic gonadotrophin, hCG)诱导卵泡发育，d6收集卵巢黄体细胞，体外培养24 h后，不加或加入不同剂量(0.1 #mu#g/mL、1 #mu#g/mL、10 #mu#g/mL) PAF，37℃、5% CO_(2)培养箱内培养24 h。用放射免疫方法测定培养液中孕酮的含量，流式细胞仪和RT--PCR方法检测黄体细胞凋亡以及VEGF mRNA的表达。结果显示，PAF促进黄体细胞孕酮分泌，1 #mu#g/mL PAF作用最强({sl P}<0.05)；黄体细胞凋亡对PAF无明显剂量依赖性，但10 #mu#g/mL PAF显著促进大鼠黄体细胞凋亡({sl P}<0.05)；PAF刺激黄体细胞VEGF mRNA表达，1 #mu#g/mL PAF效果最显著({sl P}<0.01)。结果提示，PAF可通过调节黄体细胞孕酮的分泌和VEGF mRNA的表达来促进黄体形成。

关键词： 血小板活化因子; 黄体; 血管内皮生长因子

Role of platelet--activating factor in progesterone synthesis and vascular endothelial growth factor expression in rat luteal cells

Zheng Huili, Wen Haixia, Liu Guoyi, Ni Jiang

Department of Physiology, Harbin Medical University.Harbin 150086,Heilongjiang

Abstract

The present study aimed to investigate the role of platelet-activating factor (PAF) on progesterone synthesis and vascular endothelial growth factor (VEGF) expression in rat luteal cells. Immature (25-28-day old) female Sprague-Dawley rats were injected subcutaneously with 50 IU pregnant mare serum gonadotrophin (PMSG), and 25 IU human chorionic gonadotrophin (hCG) 48 h later, to induce folliclular development. Seven days after hCG administration, the rats were killed by guillotine and the ovary luteal cells were collected. After incubation for 24 h, luteal cells were incubated without or with different doses (0.1 #mu#g/mL, 1 #mu#g/mL, 10 #mu#g/mL) of PAF at 37℃ (5% CO_(2)) for 24 h, then progesterone concentration was evaluated by radioimmunoassay (RIA); apoptotic rate and VEGF expression in luteal cells were also assessed by flow cytometry and RT-PCR, respectively. The results showed that PAF promoted progesterone production, with a maximal effect at a 1 #mu#g/mL (P<0.05); PAF increased apoptotic rate not in a dose-dependent manner, but 10 #mu#g/mL PAF enhanced apoptotic rate significantly (P<0.05); furthermore, PAF stimulated VEGF expression in luteal cells, especially at 1 #mu#g/mL (P<0.01). It is suggested that PAF regulates progesterone synthesis and VEGF mRNA expression in luteal cells to mediate corpus luteum formation in rat ovary.

Key words: platelet-activating factor;luteum;vascular endothelial growth factor

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引用本文：

郑慧丽, 温海霞, 刘国艺, 倪江. 血小板活化因子对大鼠黄体细胞孕酮分泌及血管内皮生长因子表达的作用[J]. 生理学报 2008; 60 (2): 275-278.

Zheng Huili, Wen Haixia, Liu Guoyi, Ni Jiang. Role of platelet--activating factor in progesterone synthesis and vascular endothelial growth factor expression in rat luteal cells. Acta Physiol Sin 2008; 60 (2): 275-278 (in Chinese with English abstract).