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川芎嗪抗链霉素耳毒性作用及其对豚鼠耳蜗外毛细胞钾通道的影响

汤浩, 崔桂英, 石丽娟, 高青华, 曹宇

中国医科大学基础医学院生理学教研室.辽宁,沈阳 110001

摘要

该文旨在研究川芎嗪（tetramethylpyrazine，TMP）拮抗链霉素耳毒性作用及其对豚鼠耳蜗外毛细胞K~(+)通道的影响，探讨TMP拈抗链霉素耳毒性的离子通道机制。60只豚鼠随机分为6组，应用听觉脑干反应（auditory brainstem response，ABR）技术检测豚鼠ABR听阈，观测TMP的抗链霉素耳毒作用；并采用全细胞膜片钳技术观察TMP对耳蜗外毛细胞Ca~(2+)敏感K~(+)电流的影响。结果显示，TMP明显降低链霉素导致的豚鼠ABR听阈升高，提示TMP具有抗链霉素耳毒性作用；TMP能明显增大豚鼠耳蜗外毛细胞Ca~(2+)敏感K~(+)电流，并呈浓度依赖关系。结果提示，TMP通过增大K~(+)通道电导而拮抗链霉素耳毒性作用。

关键词： 川芎嗪; 外毛细胞; 链霉素; K~(+)通道

Attenuation of streptomycin ototoxicity by tetramethylpyrazine and its effect on K~(+) channels in the outer hair cells of guinea pig cochlea

Tang Hao, Cui Guiying, Shi Lijuan, Gao Qinghua, Cao Yu

Department of Physiology, School of Basic Medical Sciences, China Medical University.Shenyang 110001,Liaoning

Abstract

In order to elucidate the mechanism underlying the attenuation of streptomycin ototoxicity by tetramethylpyrazine （TMP）, the present study investigated the effect of TMP on the outward K~(+) current in the outer hair cells of guinea pig cochlea. Sixty guinea pigs were divided into 6 groups randomly. Auditory brainstem response （ABR） was used to observe the change in thresholds and to evaluate ototoxicity induced by streptomycin. Whole-cell patch-clamp technique was used to observe the effect of TMP on outward K~(+) current in isolated outer hair cells. The results showed that TMP attenuated the threshold shift caused by streptomycin and increased the amplitudes of Ca~(2+)-sensitive K~(+) current [I_(K(Ca))] in the outer hair cells. The present data suggest that TMP displays anti-ototoxicity induced by streptomycin. The augmented amplitudes of I_(K(Ca)) of the outer hair cells induced by TMP may be one of the mechanisms underlying its ototoxicity-attenuating effect.

Key words: Tetramethylpyrazine;Outer hair cells;streptomycin;K~(+) channel

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引用本文：

汤浩, 崔桂英, 石丽娟, 高青华, 曹宇. 川芎嗪抗链霉素耳毒性作用及其对豚鼠耳蜗外毛细胞钾通道的影响[J]. 生理学报 2007; 59 (4): 534-538.

Tang Hao, Cui Guiying, Shi Lijuan, Gao Qinghua, Cao Yu. Attenuation of streptomycin ototoxicity by tetramethylpyrazine and its effect on K~(+) channels in the outer hair cells of guinea pig cochlea. Acta Physiol Sin 2007; 59 (4): 534-538 (in Chinese with English abstract).