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香烟烟雾提取物抑制肺泡上皮细胞的增殖并诱导其凋亡

焦宗宪, 敖启林, 熊密

华中科技大学同济医学院病理学系.湖北,武汉 430030

摘要

香烟烟雾提取物(cigarette smoke extract,CSE)中含有丰富的氧化剂和自由基,由它所引起的氧化应激可导致肺泡壁的损伤进而发展为肺气肿。近年来,围绕CSE损伤肺泡壁作用机制的研究较为活跃,但其结果却一直存在着分歧。该实验的目的是观察CSE对肺泡Ⅱ型上皮细胞的损伤作用并探讨与其相关的分子机制。MTT比色法的结果显示,CSE以时间和剂量依赖性的方式降低细胞的增殖活力,流式细胞术的分析结果表明细胞增殖周期被阻滞在G_(1)/S期。Hoechst 33258染色以及透射电镜观察从形态上确认CSE诱导细胞凋亡的发生,DNA梯的出现和Annexin V--FITC/碘化丙啶双染色的结果从分子水平得到进一步的证实。同时,运用流式细胞术检测到CSE诱导的凋亡伴随着Fas受体的高表达和caspase-3的显著活化。另外,使用H_2DCFDA染色,经激光共聚焦显微镜术测得细胞内氧自由基在细胞受到CSE刺激以后大量快速积累。结果表明CSE能够抑制肺泡Ⅱ型上皮细胞来源的A549细胞的生长和增殖,并诱导细胞凋亡,由Fas受体所介导的死亡受体途径参与此凋亡过程,而CSE所引起的氧化应激则可能是阻止肺泡上皮细胞生长增殖并诱导其凋亡的始动因素。

关键词： 香烟烟雾; 肺泡上皮细胞; 凋亡; Fas受体

Cigarette smoke extract inhibits the proliferation of alveolar epithelial cells and induces apoptosis

Jiao Zongxian, Ao Qilin, Xiong Mi

Department of Pathology,Tongji Medical College,Huazhong University of Science and Technology.Wuhan 430030,Hubei

Abstract

Cigarette smoke extract (CSE) contains abundant oxidants/free radicals. Oxidative stress caused by cigarette smoking results in the destruction of the alveolar cell walls and emphysema. However, there exists discrepancy about how CSE works in the process. In the present study, we observed the effect of CSE on the cell growth of alveolar epithelial cell type II-derived A549 cell line, and provided molecular understanding of this effect. The MTT assay results showed that CSE decreased the cell viability of A549 cells in a dose- and time-dependent manner, and cell cycle was arrested in G_(1)/S phase. Furthermore, CSE-induced apoptosis of A549 cells was verified by Hoechst 33258 staining, electronic microscopy in morphology, and the appearance of DNA fragmentation and annexin-FITC/propidium iodide (PI) staining assay. CSE treatment was found to result in the upregulation of Fas/APO-1 receptor and activated caspase-3. Besides, CSE also initiated accumulation of intracellular reactive oxygen species, which was detected by laser confocal microscopy. Taken together, CSE could inhibit the cell growth and induce apoptosis of A549 cells involving Fas receptor pathway, oxidative stress caused by CSE may be the radical factor leading to apoptosis as well as cell growth inhibition of alveolar epithelial cells.

Key words: cigarette smoke;alveolar epithelial cells;Apoptosis;Fas receptor

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引用本文：

焦宗宪, 敖启林, 熊密. 香烟烟雾提取物抑制肺泡上皮细胞的增殖并诱导其凋亡[J]. 生理学报 2006; 58 (3): .

Jiao Zongxian, Ao Qilin, Xiong Mi. Cigarette smoke extract inhibits the proliferation of alveolar epithelial cells and induces apoptosis. Acta Physiol Sin 2006; 58 (3): (in Chinese with English abstract).