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胃缺血--再灌注对大鼠胃黏膜细胞凋亡和增殖的影响

乔伟丽, 王琳, 张建福, 张咏梅

徐州医学院生理学教研室.江苏,徐州 221002；徐州医学院组织胚胎学教研室.江苏,徐州 221002

摘要

该研究采用大鼠胃缺血--再灌注(gastric ischemia--reperfusion,GI--R)模型(夹闭腹腔动脉30min后再灌注),通过组织学免疫组化等方法,研究GI--R不同时间(0、0.5、1、3、6、24、48、72 h)对胃黏膜细胞凋亡和增殖的影响。结果发现,单纯缺血30min胃黏膜损伤较轻,再灌注后损伤逐渐加重,胃黏膜的凋亡细胞迅速增加,而增殖细胞迅速减少：至再灌注后1h达高峰；之后胃黏膜开始修复,凋亡细胞逐渐减少,增殖细胞逐渐增加：至再灌注后24h胃黏膜细胞增殖达高峰；再灌注后72h胃黏膜基本恢复正常。上述结果提示,在GI--R中,胃黏膜损伤主要由再灌注引起,凋亡细胞增加；然后胃黏膜启动自我修复机制,增殖细胞逐渐取代损伤细胞,3d左右就可基本修复,表明胃黏膜细胞具有很强的自我修复能力。

关键词： 胃; 缺血-再灌注; 凋亡; 细胞增殖; 大鼠

Effects of gastric ischemia--reperfusion on gastric mucosal cellular apoptosis and proliferation in rats

Qiao Weili, Wang Lin, Zhang Jianfu, Zhang Yongmei

Department of Physiology,Xuzhou Medical College.Xuzhou 221002,Jiangsu；China

Abstract

The effect of gastric ischemia-reperfusion (GI-R) on gastric mucosal cellular apoptosis and proliferation was investigated using histological, immunohistochemical methods in Sprague-Dawley rats. The GI-R model was established by clamping the celiac artery for 30 min and reperfusing for 0, 0.5, 1, 3, 6, 24, 48, 72 h, respectively. Mild gastric mucosal injury was induced by ischemia alone. However, the injury was worsened and reached a maximum at 1 h after reperfusion, almost simultaneous with that the gastric mucosal cellular apoptosis was increased and cellular proliferation was decreased in gastric mucosa. Then, gastric mucosal cells began to repair by increased gastric cellular proliferation, which achieved a maximum at 24 h after reperfusion. The mucosal lesions were repaired almost completely at about 72 h after reperfusion. These results indicate that the gastric mucosal injury after GI-R is mainly induced by reperfusion. The damaged gastric mucosa could initiate its repairing mechanism immediately through inhibiting cellular apoptosis and increasing the number of proliferative cells, which substitute the damaged cells gradually. The plerosis almost completes in three days after reperfusion showing a strong self-repair ability of gastric mucosa.

Key words: stomach;Ischemia-reperfusion;Apoptosis;cellular proliferation;Rats

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引用本文：

乔伟丽, 王琳, 张建福, 张咏梅. 胃缺血--再灌注对大鼠胃黏膜细胞凋亡和增殖的影响[J]. 生理学报 2006; 58 (3): .

Qiao Weili, Wang Lin, Zhang Jianfu, Zhang Yongmei. Effects of gastric ischemia--reperfusion on gastric mucosal cellular apoptosis and proliferation in rats. Acta Physiol Sin 2006; 58 (3): (in Chinese with English abstract).