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四种不同高血压大鼠模型形成过程中血清抗AT_(1A)受体自身抗体的检测

陈蓉芳, 王瑾, 焦向英, 刘慧荣, 赵荣瑞, 支建明

第二军医大学卫生毒理学教研室. 上海 200433；山西医科大学生理学教研室. 山西，太原 030001；上海交通大学医学院生理学教研室. 上海 200025

摘要

该实验采用不同的方法复制两肾一夹(two--kidney one--clip renal hypertensive，2K1C)、神经性、DOCA--盐高血压和自发性高血压大鼠模型，观察AT1A受体自身抗体(AT_(1A)--receptor autoantibodies, AT_(1A)--AAs)在不同高血压发病的变化规律，同时对自身抗体生物活性进行分析。实验结果表明，在高血压发病过程中AT_(1A)--AAs的阳性率和滴度均明显增加，在四种模型中，自发性高血压组最明显，2K1C和神经性组次之。AT_(1A)--AAs的生物活性显示，可增加培养的新生鼠心肌细胞跳动频率和血管收缩张力。 结果提示，自身免疫机制参与了高血压的形成，AT_(1A)--AAs可能与心肌肥厚有关。

关键词： AT_(1A)受体; 自身抗体; 高血压; 大鼠

Detection of serum autoantibodies against AT_(1A)--receptor during the development of the four types of hypertensive rat models

Chen Rongfang, Wang Jin, Jiao Xiangying, Liu Huirong, Zhao Rongrui, Zhi Jianming

Department of Toxicology, the Second Military Medical University. Shanghai 200433, China；Department of Physiology, Shanxi Medical University. Taiyuan 030001, China；Department of Physiology, School of Medical Sciences, Shanghai Jiaotong University. Shanghai 200025, China

Abstract

Using two-kidney one-clip renal hypertensive (2K1C group), stress-induced hypertensive (neural group), DOCA-salt treated hypertensive (DOCA group) and spontaneously hypertensive rats (SHR group), to investigate the change in AT_(1A)-receptor autoantibodies (AT_(1A)-AAs) during the development of the four types of hypertension. The biological activities of AT_(1A)-AAs were examined. It was shown that the frequency of occurrence and titres of AT_(1A)-AAs increased significantly during the development of hypertension. In the four hypertensive groups studied, the occurrence of AT_(1A)-AAs was most prominent in SHR, 2K1C and neural groups. The biological effects of AT_(1A)-AAs were shown to increase the beating frequency of cultured neonatal myocardial and vascular contractile tension. It is suggested that autoimmune mechanisms are involved the pathogenesis of different types of hypertension and the AT_(1A)-AAs may be one of the mechanisms leading to cardiac hypertrophy.

Key words: Receptor, angiotensin, type 1;Autoantibody;;

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引用本文：

陈蓉芳, 王瑾, 焦向英, 刘慧荣, 赵荣瑞, 支建明. 四种不同高血压大鼠模型形成过程中血清抗AT_(1A)受体自身抗体的检测[J]. 生理学报 2006; 58 (1): .

Chen Rongfang, Wang Jin, Jiao Xiangying, Liu Huirong, Zhao Rongrui, Zhi Jianming. Detection of serum autoantibodies against AT_(1A)--receptor during the development of the four types of hypertensive rat models. Acta Physiol Sin 2006; 58 (1): (in Chinese with English abstract).