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发育过程中肝脏血管活性肠肽及其受体量的变化

王利, 唐承薇, 王春晖, 李献

重庆医科大学附属第一医院血液科. 重庆 400016；四川大学华西医院人类疾病生物治疗教育部重点实验室,人类疾病相关多肽研究室. 四川, 成都 610041

摘要

已有的研究观察到,胚胎肝脏中血管活性肠肽(vasoactive intestinal polypeptide,VIP)及其受体(vasoactive intestinal polypeptide receptor,VIPR)与造血干细胞生长和肝脏发育有关.该研究旨在了解发育过程中肝VIP及VIPR量的动态变化.采用放射免疫分析法、生物分子相互作用系统和RT-PCR等技术检测了各发育阶段大鼠肝组织VIP浓度、VIP受体结合量及VIP受体表达亚型,实验观察到胎鼠和新生鼠肝脏VIP浓度显著低于未成年鼠及成年鼠肝脏VIP浓度(P＜0.05).发育尚未成熟时(胎鼠、新生鼠、未成年鼠),肝VIPR表达均明显高于成年鼠(P＜0.05),表明大鼠在发育过程中肝脏VIP与VIP受体量呈相反的变化趋势.大鼠发育各时期,肝脏均表达VIPR-1.这些结果部分解释了肝脏发育、肝脏造血转移等重要生理现象。

关键词： 肝脏; 发育; 血管活性肠肽; 血管活性肠肽受体

Quantitative variation of vasoactive intestinal polypeptide and its receptor in rat liver during development

Wang Li, Tang Chengwei, Wang Chunhui, Li Xian

The First Hospital, Chongqing University of Medical Sciences. Chongqing 400016, China；Division of Peptides Related with Human Diseases, Key Laboratory of Biotherapy of Human Diseases, Ministry of Education, West China Hospital, Sichuan University. Chengdu 610041, Sichuan, China

Abstract

Accumulated data have suggested that vasoactive intestinal polypeptide (VIP) and corresponding receptor (VIPR) are involved in the development of hematopoietic stem cells and liver growth. In the present study, radioimmunoassay, biomolecular interaction analysis and reverse transcriptation polymerase chain reaction were used to quantify VIP, VIPR and detect the subtype of VIPR in rat liver during development. VIP concentration of liver in fetal or neonatal rats was significantly lower than that of teens or adult rats (P<0.05). The binding capacities of VIPR in liver of immature rats were much greater than that of the adult rats (P<0.05). The tendency of change in VIP concentration was contrary to that of the binding capacity of VIPR in the liver of rats during development. VIPR-1 was expressed in rat liver in all phases of development. These results may be of benefit to the understanding of the mechanisms of liver growth and fetal liver hemopoiesis shift.

Key words: Liver;Development;Vasoactive intestinal polypeptide;Vasoactive intestinal polypeptide receptor

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引用本文：

王利, 唐承薇, 王春晖, 李献. 发育过程中肝脏血管活性肠肽及其受体量的变化[J]. 生理学报 2005; 57 (3): .

Wang Li, Tang Chengwei, Wang Chunhui, Li Xian. Quantitative variation of vasoactive intestinal polypeptide and its receptor in rat liver during development. Acta Physiol Sin 2005; 57 (3): (in Chinese with English abstract).