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糖皮质激素快速抑制牛蛙椎旁神经节B细胞快兴奋性突触后电位

马蓓, 易必达, 邢宝仁

第二军医大学生理教研室. 上海 200433；贵阳医学院生理教研室. 贵阳 500045

摘要

用单个方波电刺激牛蛙离休椎旁神经节节前纤维，细胞内记录节后B细胞快兴奋性突触后电位（f－EPSP），观察糖皮质激素（GC）对B细胞f－EPSP，的快速抑制作用。结果发现，GC灌流3min，B细胞f－EPSP的幅值减小，撤除GC后，EPSP的幅值恢复到对照水平，作用具有剂量依赖性。糖皮质激素胞内受体阻断剂RU38486能阻断GC的快速抑制作用。蛋白质合成抑制剂放线菌酮不能阻断该快速作用。结果提示，GC对B细胞兴奋性突触后电位的快速抑制是通过非基因机制起作用的。

关键词： 糖皮质激素; 椎旁神经节; 兴奋性突触后电位; 快速作用

Abstract

In the present study, the rapid effect of hydrocortisone 21-hemisuccinate (F-suc) on the amplitude of f-EPSP of B cells in isolated bullfrog symathetic ganglia was examined with the use of intracellular recording technique. It was found tha the amplitude of f-EPSP of B cells was decreased by F-suc, with a latency less than 3 min after the beginning of perfusion of F-suc. The inhibition of F-suc was concentration-dependent and could be blocked by the antagonist of intracellular glucocorticoid receptor RU38486, but not by the protein synthesis inhibitor actidione. These findings suggest that the inhibition of f-EPSP of B cells by F-suc is probably mediated through nongenomic mechanism.

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引用本文：

马蓓, 易必达, 邢宝仁. 糖皮质激素快速抑制牛蛙椎旁神经节B细胞快兴奋性突触后电位[J]. 生理学报 1998; 50 (6): .

, , . . Acta Physiol Sin 1998; 50 (6): (in Chinese with English abstract).