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Wnt信号通路关键基因β-catenin在RA诱导的P19胚胎性癌细胞神经分化过程中的亚细胞分布

杨靖, 侯海宇, 边玮, 林其谁, 景乃禾

中国科学院上海生物化学研究所. 上海 200031

摘要

Wnt信号参与了小鼠早期神经发育。我们以往的实验结果表明，Wnt信号可引起P19胚胎性癌细胞的神经分化。为了进一步了解Wnt信号在P19神经分化过程中行使功能的时间，我们以Wnt信号通路关键成员β-catenin是否定位在细胞核中作为考察Wnt信号是否能传递到细胞核内调控下游基因活性的指标，分析了Wnt信号在P19细胞神经分化过程中的活性。我们观察到，β-catenin在RA诱导的P19细胞分化的第2到第4天存在核定位。在此期间，Wnt下游基因En－2的表达量也有明显增加。因此，在P19神经分化过程中，Wnt活性可能发生在这一阶段。

关键词： Wnt; β-catenin; P19胚胎性癌细胞; 神经分化

Abstract

Wnt signals have been shown to play an important role in the development of the central nervous system (CNS) of mouse. In our previous work, it was demonstrated that Wnt signal could initiate differentiation of P19 EC cells. In the present inveshgation, it was examined with RT-PCR whether expression of β-catenin, a downstream of Wnt in its signal triduction pathway, is regulated. It was found that the level of protein or transcript β-catenin during P19 neuronal differentiation was not changed. However,immunostaining data showed that β-catenin was translocalized into nuclei after retinoic acid induced P19 cell mggates were trypsinized and cultured in serum free N2 medium for 2 and 4 d. In this period, transcription of En-2, a downstream target gene of Wnt signal, increased evidenily. The ahove data suggest that Wnt signals are involved in the early stage of neuronal differentiation process of P19 cell.

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杨靖, 侯海宇, 边玮, 林其谁, 景乃禾. Wnt信号通路关键基因β-catenin在RA诱导的P19胚胎性癌细胞神经分化过程中的亚细胞分布[J]. 生理学报 1998; 50 (6): .

, , , , . . Acta Physiol Sin 1998; 50 (6): (in Chinese with English abstract).