过刊浏览

100Hz电针耐受时大鼠脑和脊髓k受体结合特性的改变

董艺娜, 毛慕华, 王晓民, 韩济生

北京医科大学神经科学研究所. 北京 100083；江西井冈山医学高等专科学校生理教研室. 吉安 343000

摘要

本文采用放射配体结合实验研究了100Hz电针耐受发生发展过程中大鼠脑和脊髓k受体结合特性的变化。大鼠每天给予100Hz电针1次（30min），连续7d。分别在电针的第1.3、5.7天取不同脑区进行观察。结果表明：在100Hz电针耐受期间，大鼠中脑、纹状体、脊髓.皮层和脑桥／延髓的K受体均表现为下调，但在不同脑区K受体下调的时程不同。在大鼠皮层和脑桥／延髓，电针第1天即引起k受体下调，并在全过程（7d）内保持下调状态。脊髓中的k受体先上调后下调，而中脑和纹状体k受体下调的时程与100Hz慢性电针耐受的发生发展相平行，提示该脑区的。

关键词： 电针; 镇痛; 耐受; k-阿片受体

Abstract

The effect of repetitive 100 Hz EA stimulation on the characteristics of binding of Kopioid receptors in discrete brain regions and the spinal cord of rats was investigated by radioligand binding assay. 100 Hz EA was administered once a day (30 min) for 7 d.Changes in Bmax and Kd of K opioid receptors of 4 discrete brain regions (cortex,midbrain, pons-medulla and striatum) and spinal cord were observed at d 1, 3, 5 and 7 ,respectively. It was found that the numbers (Bmax) of K opioid receptors of all the brain regions observed were decreased during the development of 100 Hz EA tolerance with a differential time course . In rat cortex and pens-medulla, a marked down regulation of Kopioid receptors occurred within 24 h and remained at al low level throughout the observation. The Bmax of K receptor in spinal cord showed a bell-shaped curve, i. e., upregulation in the first 5 days and down regulation at d 7.

Key words: ;;;

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

董艺娜, 毛慕华, 王晓民, 韩济生. 100Hz电针耐受时大鼠脑和脊髓k受体结合特性的改变[J]. 生理学报 1998; 50 (6): .

, , , . . Acta Physiol Sin 1998; 50 (6): (in Chinese with English abstract).