过刊浏览

5-HT增强大鼠骶髓后连合核神经元牛磺酸激活的全细胞电流

王殿仕, 徐天乐, 庞志平, 李继硕

第四军医大学基础部解剖学教研室, 梁銶琚脑研究中心. 西安 710032

摘要

应用制霉菌素穿孔全细胞记录方法，研究了5-羟色胺（5－HT）对急性分离的大鼠骶髓后连合核（SDCN）神经元牛磺酸（taurine，Tau）激活的全细胞电流的调控。实验结果如下：（1）在SDCN神经元，Tau作用在士的宁（strychnine，STR）敏感型甘氨酸（glycine，Gly）受体，在箝制电位为-4OmV时，可引起内向电流；（2）5－HT在001~100μmol／L范围内呈浓度依赖性地增强Tau反应（ITau）；（3）5－HT的增强作用并未改变ITau的翻转电位及Tau与Gly受体的亲和力。

关键词： 5-羟色胺; 牛磺酸; 穿孔全细胞记录; 骶髓后连合核

Abstract

Using nystatin-perforated whole-cell recording configuration, the modulatory effect of serotonin (5-hydroxytryptamine, 5-HT) on taurine (Tau)-actovated current (ITau) was investigated in neurons dissociated from the rat sacral dorsal commissural nucleus (SDCN). The results are as follows: (1)Tau acted on strychnine-sensitive glycine glycine (Gly)receptors and elicited inward currents at a holding potential (VH) of-40 mV in SDCN neurons; (2) in a range of 0.01 to 100 μmol/L, 5-HT enhanced ITau. in a concentration dependent manner; (3) both the reversal potential of Tau and the binding affinity of Tau to Gly receptor were not affected by 5-HT; (4) when the neurons were loaded with 3 μmol/L chelerythrine, application of 1 μmol/L 5-HT failed to enhance ITau. The above results thus indicate that intracellular PKC pathway is at least partially involved in the enhancement of ITau by 5-HT.

Key words: ;;;

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

王殿仕, 徐天乐, 庞志平, 李继硕. 5-HT增强大鼠骶髓后连合核神经元牛磺酸激活的全细胞电流[J]. 生理学报 1998; 50 (6): .

, , , . . Acta Physiol Sin 1998; 50 (6): (in Chinese with English abstract).