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颈动脉注射腺苷对去缓冲神经大鼠延髓腹外侧头端区神经元放电的影响

陈爽, 何瑞荣

河北医科大学基础医学研究所生理室. 石家庄 050017

摘要

在28只切断双侧缓冲神经的Sprague－Dawley大鼠，应用细胞外记录方法，观察了72个自发放电单位中颈动脉注射腺苷对延髓腹外侧头端（RVLM）区神经元自发放电活动的影响。所得结果如下：（1）颈动脉注射腺苷（25μg/kg），31个单位的放电频率由23．5±3．0下降至（16．5±2．6）spikes／s（P＜0．001），血压和心率无明显变化（P＞0．05）。

关键词： 腺苷; 延髓腹外侧头端区; 自发放电单位; 8-本茶碱; 8-环戊-1; 3-二丙基黄嘌呤; 格列苯脲

Abstract

To observe the effect of intracarotid administraation of adenosine on neurons in the rostral ventrolateral medulla (RVLM) region, 72 spontaneous active units were extracellulary recorded from 28 Sprague-Dawley rats with sino-aortic denervation. The results obtained are as follows: (1) intracarotidadministration of Ado (25 μg/kg) reduced the discharge rate from (23 .5 ± 3.0) to (16.5± 2.6) spikes/s (P< 0.001) in 31 out of 36 RVLM neurons, while the blood pressure and heart rate had no significant change (P > 0.05); (2) 8-phenyltheohylline (8-PT, 15 μg/kg), a nonselective adenosine receptor antagonist, and 8-cyclopentyl-1,3-dipropylxanthinee (DPCP, 50 μg/kg), a selective A1 adenosine receptor antagonist,completely blocked the inhibitory effects of Ado in 24 units; and (3) in 12 units, glibenclamide (500) μg/kg), a blocker of ATP-sensitive potassium channel, abolished the effect of Ado.

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引用本文：

陈爽, 何瑞荣. 颈动脉注射腺苷对去缓冲神经大鼠延髓腹外侧头端区神经元放电的影响[J]. 生理学报 1998; 50 (6): .

, . . Acta Physiol Sin 1998; 50 (6): (in Chinese with English abstract).