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低氧对肺内小动脉平滑肌细胞血管紧张素转化酶活性和基因表达的影响

于天正, 马传桃

湖南医科大学心血管生理研究室. 长沙 410078

摘要

实验采用分离培养兔肺内小动脉平滑细胞（PASMC），观察低氧对PASMC的血管紧张素转化酶（ACE）活性和基因表达的影响。结果表明：（1）正常情况下培养的兔PASMC中有ACE基因表达并可检测到其活性，而培养基中未检测到ACE活性；（2）低氧6h后，ACE基因表达变化不明显，但在随后的12、24、48h后表达明显增加，呈时间依赖性；（3）低氧24h后，PASMC细胞中及条件培养基中ACE活性均显著增加。上述结果提示：（1）基础状态下培养的免PASMC存在ACE；（2）低氧可促进PASMCACE活性和基因表达，这可能与低氧性肺动脉高压有关。

关键词： 低氧; 肺动脉; 平滑肌细胞; 血管紧张素转化酶（ACE）; 兔

Abstract

In other to observe the effect of hypoxia on pulmonary artery smooth muscle cells (PASMC), angiotensin-converting enzyme (ACE) activity was measured by colometry and the expression of ACE mRNA was detected by Northern blot, using rabbit isolated and cultured PASMC. The main results are as follows: (1) under normoxia both ACE activity and gene expression could be detected in PASMC; and (2) the expression level of ACE mRNA, after an initial latent period of 6 h hypoxia, began to show a marked increase with time in the next 12, 24 and 48 h. Exposure to hypoxia for 24 h resulted in an increase in either culture medium or cell ACE activity, as compared with that under normoxia. The results suggest that PASMC ACE increased by hypoxia might play an important role in the development of hypoxic pulmonary hypertension.

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引用本文：

于天正, 马传桃. 低氧对肺内小动脉平滑肌细胞血管紧张素转化酶活性和基因表达的影响[J]. 生理学报 1998; 50 (6): .

, . . Acta Physiol Sin 1998; 50 (6): (in Chinese with English abstract).