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一氧化氮及去内皮对颈动脉窦压力感受器活动的影响

夏林英, 钮伟真, 沈靖, 刘北英

首都医科大学心血管研究室. 北京 100054

摘要

本实验应用自制的颈动脉窦（CS）窦内脉动灌流-窦外表面灌流的双重灌流装置，研究了外源性一氧化氮（NO）及内皮对家兔离休CS压力感受器活动（BRA）的影响，并进一步探讨其作用机制。实验表明：（1）在脉动式灌流的条件下，卖神经（CSN）传入纤维基础放电中的高幅值放电呈现显著的对脉动式灌流的依赖性和对灌流压（平均压）依赖性，而对经典的化学感受器刺激无反应，这些放电特征证明其来自CS压力感受器；（2）去除血管内皮后，压力感受器活动减少。

关键词： 压力感受器; 一氧化氮; 内皮; 颈动脉窦; 家兔

Abstract

The present study was undertaken to examine the effects of endothelium and exogenous NO on the baroreceptor discharge of the carotid sinus. The baroreceptor activity (BRA) was recorded from the rabbit carotid sinus-sinus nerve (CS-CSN) preparation using a chamber for superfusing and perfusing the tissue. The results are as follows. (1)The CSN hasal discharge could be divided into two components, a low-amplitude component and a high-amplitude one. The high-amplitude discharge was dependent on pulsatory perfusing pressure, but did not respond to classical chemoreceptor stimulants,indicating its baroreceptive origin. (2) The BRA was decreased after removal of the endothelium. (3 ) L-arginine (L-arg), as exogenous NO, initiated BRA in a dosedependent manner, which was prevented by precedent inhibition of NO synthase or by removalof the endothelium. (4) Inhibition of guanine nucleotide cyclase with methylene blue effected neither the hasal BRA nor the response of BRA to L-arg.

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引用本文：

夏林英, 钮伟真, 沈靖, 刘北英. 一氧化氮及去内皮对颈动脉窦压力感受器活动的影响[J]. 生理学报 1998; 50 (6): .

, , , . . Acta Physiol Sin 1998; 50 (6): (in Chinese with English abstract).