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内源性阿片物质参与大鼠缺血预处理的心肌保护作用

傅李莉, 夏强, 沈岳良, 黄德明

浙江医科大学生理学教研室. 杭州 310031；香港大学医学院生理系. 香港

摘要

实验以离休灌流的SD大鼠心脏为模型，用k特异性桔抗剂MR2266研究k阿片受体的阻断与缺血预处理（IP）的关系，用放射免疫分析法研究IP及长时间缺血对心肌强啡肽A1-13（DynA1－13）浓度的影响，探索K阿片物质在IP过程中的作用和地位。结果显示：（1）IP可减轻缺血／复灌性心律失常严重程度（P＜0．05），缩小心肌梗死范围（P＜0．01），对冠脉流量和心率无明显影响;（2）MR2266可减轻缺血／复灌性心律失常严重程度（P＜0．05），缩小心肌梗死范围（P＜0．01），促进复灌过程中冠脉流量的恢复，对心率无明显影响。

关键词： 阿片物质; 缺血预处理; 心律失常

Abstract

In the present stUdy, the relationship between the blockade Of K-opioid receptor and ischemic preconditioning (IP) was examined and the effect of lP and prolonged ischemia on levels of dynorphin A1-13 (Dyn A1-13) in cardiac muscle in isolated perfused rat heart was investigated. The results are as follows: (1) IP reduced the severity of ischemia/reperfusion arrhythmia (P < 0.05) and infaret size (P < 0.01 ), but had no significant effect on heart rate and coronary flow (p > 0.05); (2) MR2266, K opioid receptor antagonist, reduced the severity of ischemia/reperfusion arrhythmia(P < 0.05)and infarct size (P < 0.01 ), and also enhanced the recovery of coronary flow, but had no significant effect on heart rate (P > 0.05); and (3) prolonged ischemia decreased the levels of Dyn A1-13 (P < 0.05), which was more marked in the unpreconditioned hearts. The results suggest: (1 ) MR2266 can "mimic" cardioprotective effect of IP in reducing the severity of arrhythmias and limiting infarct size of cardiac muscle.

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引用本文：

傅李莉, 夏强, 沈岳良, 黄德明. 内源性阿片物质参与大鼠缺血预处理的心肌保护作用[J]. 生理学报 1998; 50 (6): .

, , , . . Acta Physiol Sin 1998; 50 (6): (in Chinese with English abstract).