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吗啡对福尔马林引起大鼠海马内IL-2RβmRNA表达的影响

吴旋, 黎海蒂, 李希成, 阮怀珍, 王建

第三军医大学生理教研室. 重庆 400038

摘要

本实验采用原位杂交法观察足底注射福尔马林（For）痛敏对海马内白细胞介素2受体βmRNA（IL-2RβmRNA）生成的影响及其与吗啡、促肾上腺皮质激素（ACTH）的关系。结果表明：正常大鼠海马有IL-11．2RβmRNA表达，集中分布于CA1－CA4区神经元、齿状回颗粒细胞。足底注射For后6h，双侧海马IL2RβmRNA表达均增加（P＜0．05），12h达高峰，24h仍高于正常。在6h时，腹腔注射吗啡可大幅度的提高For引起的IL-2RβmRNA表达增多效应，而腹腔注射ACTH却无此类似作用。提示海马内IL-2R可能与痛觉调制有关。

关键词： 白细胞介素2受体βmRNA; 海马; 吗啡; 促肾上腺皮质激素; 原位杂交

Abstract

Using mothod of in situ hybridization, the effects of subcutaneous injection (sc) offormalin (For) into one hindpaw (hyperalgesia) on IL-2R β mRNA expression inhippocampus (Hip) were studied. In addition, the effects of coadrinistiation ofmorphine (ip) or ACTH (ip) with For (sc) were studied also. It was found that IL-2Rβ mRNA was present in nounal rat's Hip, intensely in granular cells of dentate gyrus andCA1-CA4 neurons. Subcutaneous injection of For increased IL-2RβmRNA from 6 h to atlaest 24 h, reaching a peak at 12 h. Coadministration with morphine enhanced the effectsof For on IL-2R β mRNA expression, but no significant change was found withcoadministration of ACTH. The above results suggest that IL-2R might be involved in painmodulation.

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引用本文：

吴旋, 黎海蒂, 李希成, 阮怀珍, 王建. 吗啡对福尔马林引起大鼠海马内IL-2RβmRNA表达的影响[J]. 生理学报 1998; 50 (5): .

, , , , . . Acta Physiol Sin 1998; 50 (5): (in Chinese with English abstract).