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迷路损伤增强大鼠前庭内侧核生长相关蛋白mRNA水平

孙久荣, 余瑞元, 刘文胜, 王燕峰

北京大学生命科学学院. 北京 100871

摘要

前庭代偿是研究神经可塑性的一个理想模型。生长相关蛋白（GAP－43）在神经再生和突触重组中起重要作用。用DIG（digoxigenin）标记的GAP－43cDNA片段作探针进行原位杂交，检测了大鼠迷路损伤5、12、20和30d后前庭内侧核GAP－43mDRNA表达的变化。结果表明，迷路损毁后两侧前庭内侧核GAP－43mRNA的水平以不同的幅度和时程明显升高。这一结果表示，GAP-43mRNA水平的提高可能与前庭代偿中突触重组和神经可塑性相关。

关键词： 生长相关蛋白（GAP-43）; 前庭代偿; 神经可塑性; 原位杂交

Abstract

Vestibular compensation is the most extensively investigated model forneuroplasticity. Growth-associated protein (GAP-43) mRNA plays a significant role innerve regeneration and synaptic remodeling. Using in situ hybridization with DIG(digoxigenin)-labeled GAP-43 cDNA probe, changes of GAP-43 mRNA expression inthe medial vestibular nucleus in the labyrintheectomized rats at 5, 12, 20 and 30th dayafter operation were investigated. The results clearly demonstrated that labyrinthectomyincreased GAP-43 mRNA expression to different extents and amplitudes in bilateral medialvestibular nuclei. This finding suggests that upregulation of GAP-43 mRNA expression isrelated tO regenerative sprouting, synaptic remodeling and neuroplasticity in vestibularcompensation.

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引用本文：

孙久荣, 余瑞元, 刘文胜, 王燕峰. 迷路损伤增强大鼠前庭内侧核生长相关蛋白mRNA水平[J]. 生理学报 1998; 50 (5): .

, , , . . Acta Physiol Sin 1998; 50 (5): (in Chinese with English abstract).