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糖皮质激素快速抑制高钾离子诱导嗜铬细胞瘤细胞内游离钙浓度升高的作用及其特征

娄淑杰, 王晨光, 黄秀英, 陈宜张

第二军医大学生理学教研室；神经科学研究所. 上海 200433；中国科学院发育生物学研究所开放实验室. 北京 100081

摘要

本研究应用钙离子特异荧光指示剂Fura－2／AM，使用Miracal影像系统（MiracalImageSystem）检测了糖皮质激素对高钾离子升高嗜铬细胞瘤细胞（PC12细胞）内游离钙浓度（[Ca2+]i）作用的影响。结果表明：（1）皮质酮抑制高钾离子诱导PC12细胞[Ca2+]i升高与其预处理细胞时间的长短有关，预处理3min时，皮质酮开始产生抑制作用；预处理5min时，其呈现的抑制作用最强；预处理25min时，抑制作用基本消失。

关键词： 糖皮质激素; PC12细胞; 细胞内游离钙离子浓度; 高钾离子; Fura-2／AM

Abstract

The effect of glucocorticoid on the increase of cytosolic free calcium [ Ca2+ j, )induced by high-K+ was investigated in pheochomocytoma PC12 cells. The aden restiltwas as follows: (l ) When the cells were preincubated at 37t for various time intervallin the presence of 10-5 moVL of corticosterone and stimulated with 55 mrnol/L KCI, anichbitory effect of conicosterone on b [ CaZ + ]. was observed in a bine-dependent mannerwith ~rnal effect at sib adn and no effect at 25th adn. (2) adn PC12 cells werepreincubated with various concentration of corticosterone at 37T for 5 adn, the inhibitoryeffect of corticosterone on A [ Ca2+ ]. induced by 55 rnmol/L KCI was concentrationdependent. The effect was found to be maximal at 10--5 moVL of colticosternne anddiSappeared at 10-9 moUL of corticosterone.

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引用本文：

娄淑杰, 王晨光, 黄秀英, 陈宜张. 糖皮质激素快速抑制高钾离子诱导嗜铬细胞瘤细胞内游离钙浓度升高的作用及其特征[J]. 生理学报 1998; 50 (5): .

, , , . . Acta Physiol Sin 1998; 50 (5): (in Chinese with English abstract).