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肝刺激因子对肝癌细胞增殖的调节作用

刘晓军, 安威, 雷天光, 戎煜, 杜国光

首都医科大学细胞生物系. 北京 100054

摘要

初断乳雄性SD大鼠的肝匀浆以超速离心和柱层析法分离纯化肝刺激因子（HSS），观察其对肝癌细胞增殖、细胞表皮生长因子（EGF）受体表达及受体磷酸化的影响。结果表明，HSS具有明显的促肝癌细胞分裂增殖能力，提高细胞周期中S期细胞所占比例。HSS促肝癌细胞增殖作用与其促EGF受体表达有关，表现为：（1）HSS上调170kDEGF受体蛋白表达，此作用与EGF合用后明显加强，即是协同效应；（2）HSS上调EGF受体表达呈剂量-效应和时间-效应关系;（3）HSS可刺激EGF受体酪氨酸残基的磷酸化，调节受体活性。

关键词： 肝刺激因子; 表皮生长因子; 肝细胞增殖

Abstract

The hepatic stimulator substance (HSS) was prepared from weanling rat livers byultracentrifugation and Sephadex G-75 chromatography. The stimulatory effects of HSS oncell proliferation, EGF receptor expression and receptor tyrosine phosphorylation wereinvestigated in serum-free cultured human hepatoma cell line SMMC-7721. The resultsshowed: (1) The relative percentage of S-phase cells was increased in HSS-treated cells.(2) EGF receptor protein level was increased after the cells were beated with HSS for 12~24 h and this effect was dose- and time-dependent. (3) Treatment of the cells withHSS for 0. 5 ~ 15 min resulted in the autophosphorylation of EGF receptor tyrosine residuedramatically. The above results suggest that the proliferative action of HSS on hepatocytesfright be due to its modulation of EGF receptor expression and the signaling pathway.

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引用本文：

刘晓军, 安威, 雷天光, 戎煜, 杜国光. 肝刺激因子对肝癌细胞增殖的调节作用[J]. 生理学报 1998; 50 (5): .

, , , , . . Acta Physiol Sin 1998; 50 (5): (in Chinese with English abstract).