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一种分离大鼠窦房结自律细胞的简单方法

曾英明, 李澈

汕头大学医学院生理教研室

摘要

介绍一种用胶原酶分离和初步鉴定大鼠窦房结起搏细胞的方法。操作简单方便，用酶量少。分离的起搏细胞与原位窦房结起搏细胞具有相类似的动作电位特点：有明显舒张期自动去极化，最大舒张电位平均-55mV，动作电位幅度平均58mV，APD50平均18ms，APD90平均29ms。为进一步的电生理学和组织化学研究提供适当标本。

关键词： 窦房结; P细胞; 心肌细胞; 胶原酶; 大鼠

Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

A method of isolating P cells of rat sinoatrial node by collagenase is described. The method is feasible for morphological and electrophysiological investigation, with the advantages of taking a relatively easy procedure and consuming a small amount of collagenase. The main features of action Potentials of these isolated cells including clear diastolic automatic depolarization, mean maximal diastolic potential of -55 mV, mean peak potential value of 58 mV, and mean APD50 of 18 ms, were similar to those of P cells In situ.

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引用本文：

曾英明, 李澈. 一种分离大鼠窦房结自律细胞的简单方法[J]. 生理学报 1998; 50 (4): .

Cui Hong. . Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).