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降钙素基因相关肽放射免疫测定中与内毒素交叉免疫的离体实验分析

孙威, 邢立愚, 唐跃明, 王宪

北京医科大学第三医院血管医学研究所

摘要

本文用放射免疫法分析了内毒素对降钙素基因相关肽（CGRP）离体免疫测定的影响。高浓度的内毒素（终浓度大于5μg／ml）可通过与CGRP多克隆抗体竞争性结合，影响CGBP的测定，内毒素与CGRP的交叉兔疫比率为5．6×10=6。以C18柱纯化待测样本可以有效地清除混杂的内毒素。结果表明离体实验中高浓度内毒素可干扰CGRP的放射免疫测定，C18柱可清除样本中的内毒素从而避免其对CGRP放射免疫测定的影响。

关键词： 内毒素; 降钙素基因相关肽; 放射免疫测定

Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

In the Present study,the effect of endotoxin on the radioimmununoassay of calcitonin gene-related peptide(CGRP)was investigated by method of radioimmunoassay. The results showed a competitve combination of endotoxin at high-concentrations (higher than 5μg/ml) with multiclonal antibody of CGRP, and the cross immunoreaction ratio between CGRP and endotoxin was 5 .6 ×10-6. The mixed endotoxin in samples was effectively cleared through C18 column purification. The data indicated that endotoxin interfered CGRP radioimmunoassay in vitro at high concentrations. The influence of endotoxin on CGRP edoimmunoassay can be avoided through C18 colunm Purification for the endotoxin-containing samples.

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引用本文：

孙威, 邢立愚, 唐跃明, 王宪. 降钙素基因相关肽放射免疫测定中与内毒素交叉免疫的离体实验分析[J]. 生理学报 1998; 50 (4): .

Cui Hong. . Acta Physiol Sin 1998; 50 (4): (in Chinese with English abstract).