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γ-氨基丁酸诱发人精子顶体反应及其受精能力

袁玉英, 何春娜, 石其贤

浙江省医学科学院；中国人民解放军空军454医院

摘要

本文的目的是为了探讨γ-氨基丁酸（GABA）是否可激发人精子顶体反应（AR）。受精能力及其可能的作用方式。实验采用金霉素荧光染色法（CFC）、胞内游离Ca2＋测定和精子穿透去透明带仓鼠卵（SPA）试验，分别评价GABA诱发人精子AR及其穿卵能力。结果表明：CABA可诱发人获能精子发生AR，且随精子获能进程而显著增加，并存在明显的量效关系，该作用可被P4加强。GABA还可明显地激发胞内钙［Ca2＋］i升高。然而，GABA诱发精子AR不仅可被Ca2＋螫合剂EGTA所桔抗，而且为Ca2＋通道阻滞剂La3＋所阻断。此外，GABA还可明显地促进人精子的穿卵能力。据此推论，GABA通过Ca2＋介导，调节人精子AN，促进受精率提高。上述结果为激活精子表而GABAA受体及其引起信息传递的研究奠定了基础。

关键词： GABA; 孕酮; 精子; 顶体反应; 精子穿卵试验; 胞内钙测定

Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

The present study was undertaken to investigate whether GABA induced the acrosome reaction (AR) and fertilizing ability, as well as its POssible mode of action in human spermatozoa. Spermatozoa from fifteen health fertile men isolated by the swim-up techniqUe were preincubated in a modified Bac with 0.35% BSA for 1-11 h under 5% CO2 in 95% air at 37℃. Aliquots of spermatozoa were collected at 0, 1, 3, 5,7, 9 and 11 h of incubation for evaluation of foe AR by chlortetracycline (CTC)staining. The sperm penetration assay (SPA) was carried out by using the zona-free hamster oocyte test. Intracellulax calcium ([ Ca2+ ]i ) concentrations were detendned by means of fluorescent probe Frua-2/AM. GABA at 1 .25 Umol/L significantly induced the AR in human spermatozoa preincubated for 3 h, with a maximal response in preincubated for 9 h, and the effect changed in a concentration- dependent manner.

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引用本文：

袁玉英, 何春娜, 石其贤. γ-氨基丁酸诱发人精子顶体反应及其受精能力 [J]. 生理学报 1998; 50 (3): .

Cui Hong. . Acta Physiol Sin 1998; 50 (3): (in Chinese with English abstract).