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容量负荷性肥厚心肌中血管紧张素Ⅱ-1型受体mRNA表达的动态变化

李永勤, 高广道, 楚雍烈, 巩晓宏

西安医科大学病理生理教研室；西安医科大学微生物教研室

摘要

本实验采用腹腔动静脉瘘制造大鼠容量负荷增加所致心肌肥厚模型，应用反转录-聚合酶链式反应（RT－PCR）及同位素掺入技术，检测手术后不同时间点左、右心室（LV、RV）组织中AngⅡ－1型受体的a亚型（ATla）及b亚型（ATlb）mRNA的表达。结果表明，术后早期（术后3d组）虽反映心肌肥厚的心／体重比指标已有显著性升高，而LV和RV组织的ATlamRNA及ATlbmRNA表达尚未见显著性改变。术后12d组，随着心肌肥厚的加重，RV的ATlamRNA与ATlbmRNA分别升高62．6％及720％；LV的ATlamRNA表达与假手术对照组（SO）相比升高79％，而ATlbmRNA无显著性变化。术后35d组，RV组织的ATlamRNA与ATlbmRNA分别高出对照组700％及839％，LV组织的ATlamRNA与ATlbmRNA分别高出对照组965％及869％。术后12d组和35d组，两心室组织中ATlamRNA表达均高于ATlbmRNA。心肌ATlmRNA的表达与心肌肥厚程度高度正相关。上述结果提示：ATla与ATlbmRNA表达的上调，可能是容量负荷增加所致中、后期肥厚心肌对AngⅡ反应性增强的重要机制。而ATla与ATlb两种亚型mHNA在容量负荷性肥厚心室组织中的不同表达，可能与其分别介导AngⅡ不同的生理、病理作用有关。

关键词： 心肌肥厚; 血管紧张素Ⅱ; 受体

Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

Using reverse transcriptase - polymerase chain reacti on (RT- PC R ) the changes of the angiotensin Ⅱ receptor suhtype 1-a (AT la) and 1-b (AT1b) mRNA levels were examined in hypertrophied ventricles caused by ventral aorta-caval fistula of SD rats. The results show as follows. (1) Three days after operation, the ventricular weight increased significantly, Whereas the level of ATla and A7lb InRNA in both ventricles remained unchanged. (2) Twelve days after operation, the weight increase of both ventricles was more evident, the levels of ATla and ATlb InRNA in the right ventricle increased (by 62.6% and 72.0% ), as compared with control. In the left ventricle, on the other hand, the level of ATla mRNA increased by 79.0%, while the level of ATlh mRNA showed no obvious increase. (3) mrly-five days after operation I the levels of ATla and ATlb mRNA in both ventricles increased still more significant, (ie, 70. 0% and 83. 9%in the right and 96.5% and 86.9% in the left).

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引用本文：

李永勤, 高广道, 楚雍烈, 巩晓宏. 容量负荷性肥厚心肌中血管紧张素Ⅱ-1型受体mRNA表达的动态变化 [J]. 生理学报 1998; 50 (3): .

Cui Hong. . Acta Physiol Sin 1998; 50 (3): (in Chinese with English abstract).