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外周P物质受体介导伤害性刺激所致脊髓背角c-fos基因的表达

张怡泓, 杨鲲, 李云庆, 施际武

第四军医大学基础部解剖学教研室、梁琚脑研究中心

摘要

用免疫组化染色方法，观察了P物质受体在外周对伤害性刺激信息的介导作用。于福尔马林注入双侧后肢足底前10min，将不同浓度（10-4，10-5和10－6mol／L）的SP受体特异性拮抗剂L668，169注入一侧足底，另一侧注入生理盐水。结果：10-4mol／L的L668，169明显抑制了该侧脊髓背角浅层（Ⅰ、Ⅱ层）c-fos基因的表达，而对深层影响不大；注入浓度为10－5mol／L和10－6mol／L的L668，169对背角深、浅层的作用均不明显。另外，将SP受体激动剂Sar－SP（10-4mol／L）或福尔马林分别注入不同动物的一侧后肢足底，另一侧注射等量的生理盐水，均可引起激动剂或福尔马林注入侧脊髓背角深、浅层的FOS阳性神经元数量的增多。上述结果提示，SP在外周具有致痛作用，该作用至少部分是通过SP与SP受体结合而参与介导伤害性信息向脊髓传递。

关键词： P物质; P物质受体; c－fos; 脊髓背角; 伤害性刺激; 大鼠

Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

Employing imrnunohistochendcal staining for FOS protein, which is the eXpression product of c-fos proto-oncogene, the role of substance P receptor (SPR) in noxious information transgression at peripheral terminals of the dorsal root ganglion cells was investigated by means of a selective SPR antagonist In68, 169 and agonist Sar-SP.Unilateral injection of to68, 169 (10-4 mo;/L) into the plantal of hindpaw 10 min before injection of 4% formalin, the number of FOS-like immunoreactive (LI) neurons in the superficial ldrinae of dosal horn was significantly decreased, but the changes were not obvious in the deeper laminae. Smaller doses of L668, 169 (10-5, 10-6 mol/L) were almost ineffective on FOS-LI neurons in both the superficial and the deeper laminae.However, Sar-SP (10-4 mol/L) or formalin did result in the expression of c-fos gene in the superficial laminae of the dosal horn.

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引用本文：

张怡泓, 杨鲲, 李云庆, 施际武. 外周P物质受体介导伤害性刺激所致脊髓背角c-fos基因的表达 [J]. 生理学报 1998; 50 (3): .

Cui Hong. . Acta Physiol Sin 1998; 50 (3): (in Chinese with English abstract).