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Zn~(2+)对爪蟾卵母细胞表达鲫鱼脑GABA受体的调制作用

白小川, 包永德

中国科学院上海生理研究所

摘要

爪赡卵母细胞注射鲫鱼（Caressescaresses）脑mRNA后表达的CABA受体中约85％为GABAA受体。约15％的成分为GABAC受体。本文利用双电极电压箝方法结合药物灌流研究了Zn2＋对这两型受体的作用。我们观察到Zn2＋对它们的调制都是抑制性的、可逆的。然而，与GABAA受体相比，GABAC受体对Zn2＋不太敏感。其中Zn2＋抑制鲫鱼脑GABAA受体的IC50＝48．4±10．1μmol／L，而它调制GABAC受体的IC50却高达255．6±21．5μmol／L。

关键词： Zn~（2＋）; GABA_A受; GABAc受体; 鲫鱼脑mRNA; 爪蟾卵母细胞

Cui Hong

First Clinical Institute，China Medical University. Shenyang 110001,Liaoning, China

Abstract

Two types of GABA receptors, GABAA receptors (approximately 85 % ) and GABAClike receptors (approximately 15 % ), were expressed in Xenopus oocytes after injection of Poly(A) + RNA With cap (Carassius carassius ) brain. Using voltage-clamp technique and pharmacological methods, the effects of zinc on the two receptors were studied. It was found that zinc modulation of both GABAA and GABAc-like receptors by zinc was inhibitory and reversible with the former being stronger than the latter as indicated by a value of IC5o of 48 .4 ±10.1μmol/L against 255. 6±21. 5 μmol/L.

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引用本文：

白小川, 包永德. Zn~(2+)对爪蟾卵母细胞表达鲫鱼脑GABA受体的调制作用 [J]. 生理学报 1998; 50 (3): .

Cui Hong. . Acta Physiol Sin 1998; 50 (3): (in Chinese with English abstract).