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过氧化氢诱发的小脑皮质凋亡神经元膜K~+通道电流的变化

迟先煊, 冯鉴强, 陈培熹

中山医科大学生理教研室. 广东 510089

摘要

用新生SD大鼠小脑皮质细胞进行培养，用Ara－C抑制非神经元生长，以H_(2)O_(2)诱发神经元凋亡。用膜片精细胞贴附式观察了凋亡神经元膜钾离子通道电流的变化，结果表明，凋亡小脑皮质神经元股K~(+)通道在不同箝位电压下，通道电流[I_(K)]幅度小于正常神经元的，单位电导小于正常神经元的，通道的平均开放时间、开放概率、短开放及长开放时间常数亦均小于正常神经元的。说明小脑皮质凋亡神经元K~(+)通道活动减弱。

关键词： 小脑皮质神经元; 凋亡; H_(2)O_(2)

Changes of k~(+) channel current in cerebellar cortical neuron of apoptosis induced with H_(2)0_(2)

Chi Xianxuan, Feng Jianqiang, Chen Peixi

Department of Physiology，Sun Yat-Sen University of Medical Science. Guangzhou 510089,China

Abstract

The neurons dissociated from the cerebellar cortex of neonatal SD rat were cultured,while the growth of non-neuron cells were suppressed hy Ara-c. Apoptosis of the neurons were induced with H_(2)O_(2). The changes of I_(k) were studied during apoptosis using the patch clamp technique in cell-attached confgurations. The results are as follows: (1) the Ik amplitudes of the neurons were lower than that of normal at various corresponding clamp voltages; (2) the conductances, mean open time,open probability, long and short opening time constants were all lower, as compared with normal neurons. All these results indicate that the activities of K~(+) channels on cerebellar neurons decrease during apoptosis.

Key words: Cerebellar cortical neuron;Apoptosis;H_(2)O_(2)

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引用本文：

迟先煊, 冯鉴强, 陈培熹. 过氧化氢诱发的小脑皮质凋亡神经元膜K~+通道电流的变化 [J]. 生理学报 1998; 50 (2): .

Chi Xianxuan, Feng Jianqiang, Chen Peixi. Changes of k~(+) channel current in cerebellar cortical neuron of apoptosis induced with H_(2)0_(2). Acta Physiol Sin 1998; 50 (2): (in Chinese with English abstract).