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血小板激活时胞质游离钙浓度与两种颗粒数变化的相关性

丁舰, 俞彰, 戎大梅, 钟慈声

上海医科大学生物物理学教研室. 上海 200032；上海医科大学仪器分析中心实验室. 上海 200032

摘要

用电镜形态计量法检测血小板α颗粒(αG)和致密颗粒(dG)的数密度，用钙荧光指示剂Fura2检测血小板胞质游离Ca~(2+)浓度（[Ca~(2+)]_(i))，观察到在钙离子导体A23187作用下，血小板[Ca~(2+)]_(i)明显升高。凝血酶与ADP也都分别引起[Ca~(2+)]_(i)升高，且有浓度依赖性。选用三种激动剂的不同量以反映血小板不同程度激活时，测定[Ca~(2+)]_(i)与颗粒数密度，分析两者间的相关性，发现αG和dG的数密度都与[Ca~(2+)]_(i)有紧密的相关性(P_(r(αG))＜0.05，p_(r(dG))＜0.01)，说明血小板的[Ca~(2+)]_(i)升高可促进αG和dG内容的分泌。

关键词： 血小板; α颗粒; 致密颗粒; 胞质Ca~(2+); Fura2

The relationship between cytosolic Ca~(2+) and two kinds of granule number in activated platelets

Ding Jean, Yu Zhang, Rong Damei, Zhong Cisheng

Department of Biophysics，Shanghai Medical University. Shanghai 200032,China

Abstract

The numerical density (Nv) of the #alpha#-granule (#alpha#G) and dense grantile (dG)measured by using electron dricroscopic morphometry and the cytosolic free calcium concentration ([Ca~(2+)]_(i)) by using Ca-fluorescent indicator were observed for studying the relationship of [Ca~(2+)]_(i) and Nv of αG and dG in platelets activated by A23187, thrombin or ADP. The results showed that the [Ca~(2+)]_(i) rose markedly by A23187. Thrombin or ANP could also induce an increase of [Ca~(2+)]_(i) concentration-dependently. Under 3 different degrees of activation induced by different agonists,the close relationship between Nv of both granules, #alpha#G and dG, and [Ca~(2+)]_(i) in platelets was found (P_(r(#alpha#G))< 0.05,P_(r(dG))<0.01). These findings indicate that the increase in [Ca~(2+)]_(i) may enhance the secretion of #alpha#G and dG.

Key words: Platelet;#alpha#-granule;Dense granle;Cytosolic Ca~(2+);Fura2

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引用本文：

丁舰, 俞彰, 戎大梅, 钟慈声. 血小板激活时胞质游离钙浓度与两种颗粒数变化的相关性[J]. 生理学报 1998; 50 (2): .

Ding Jean, Yu Zhang, Rong Damei, Zhong Cisheng. The relationship between cytosolic Ca~(2+) and two kinds of granule number in activated platelets. Acta Physiol Sin 1998; 50 (2): (in Chinese with English abstract).