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新生大鼠海马分区培养神经元对缺氧反应的差异

要航, 黄燕华, 刘振伟, 万勤, 丁爱石, 赵彬, 范明, 王福庄

军事医学科学院基础医学研究所神经生物学研究室

摘要

本文以混合培养海马神经元技术为基础，通过改进解剖方法、摸索鼠龄与存活率之间的关系，成功地进行了海马神经元的分区培养。同时采用同视野跟踪记数法、激光扫描共聚焦显微镜测钙和原位杂交等技术，研究了缺氧条件下培养的海马CA1和DG神经元在存活率、细胞内游离钙和脑源性神经营养因子（BDNF）mRNA表达水平等指标上变化的差异。结果表明，在相同的缺氧条件下，DG细胞比CAl细胞损伤轻微并且具有比CAl细胞更强的维持细胞内Ca2＋稳态和表达BDNFmPNA．的能力。

关键词： 海马; 缺氧; ［Ca2＋］i 脑源性神经营养因子

MA Yong-Jie

2College of Information Engineering, Taishan Medical University, Taian 271016, China

Abstract

Tissue culture from hippocmpal CA1 or dentate gyrus(DG)region was established on the basis of previous neuronal culture technique.The viability,intracellular calcium concentration and brain-derived neurotrophic factor(BDNF)mRNA expression of the two kinds of neurons after anoxia were observed in the counting in the confocal microscopic field and in situ hybridization.It is found that DG neurons are not only more resistant to anoxia,but also have a stronger ability to keep calcium homeostasis and to express BDNF mRNA than CA1 neurons.

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引用本文：

要航, 黄燕华, 刘振伟, 万勤, 丁爱石, 赵彬, 范明, 王福庄. 新生大鼠海马分区培养神经元对缺氧反应的差异 [J]. 生理学报 1998; 50 (1): .

MA Yong-Jie. . Acta Physiol Sin 1998; 50 (1): (in Chinese with English abstract).