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促乳素抑制培养的大鼠颗粒细胞中卵泡素介导的组织纤溶酶原激活因子的表达

刘以训, 柳海珍, 陈毅军, TorNY

中国科学院动物研究所生殖生物学国家重点实验室

摘要

本文主要是观察促乳素（PRL）是否影响体外培养的大鼠颗粒细胞中，组织纤溶酶原激活因子（tPA）和1型纤溶酶原激活因子抑制因子（PAⅠ-Ⅰ）基因表达间的协调作用。我们采用了多种方法，例如SDS－PAGE、免疫印迹等，来检测PRL对tPA和PAI－I基因表达的作用。结果证实：（1）在离体条件下促乳素（PRL）能刺激颗粒细胞（GC）中PAⅠ－ⅠmRNA的合成，而FSH无此作用。但FSH可与PRL协同增加GC中PAⅠ－ⅠmRNA的产生。培养到48h，PRL和FSH协同增加GC中PAⅠ－ⅠmRNA含量是PRL单独作用的7．8倍，并且显示出明显的剂量和时间依赖关系；（2）PRL或PRL加FSH对GC中PAⅠ－ⅠmRNA合成的刺激作用，与细胞分泌到培液中的PAⅠ－Ⅰ活性的增加完全一致；（3）与此相反，PRL能显著抑制FSH所诱发的GC中（tPA）mRNA和蛋白的合成，并且也有剂量依赖关系。同时发现PRL对FSH诱发的tPA活性的抑制作用与培液中PA－PAⅠ－Ⅰ复合物形成量的增加相符，这说明tPA活性的下降与PAⅠ．Ⅰ对其的中和作用有关。

Li Wenjue

Department of Physiology, Shanghai College of Traditional Chinese Medicine. Shanghai, China

Abstract

This study was designed to investigate whether prolactin(PRL)affects coordinated regulation of tissue plesminogen activator(tPA)and plasminogen activator inhibitor type-I(PAI-I)gene exprssion in rat granulosa cells in vitro.Several methods,such as SDS-PAGE,immunoblot etc.were used to detect the effect of PRL on tPA and PAI-I gene expression.The results demonstrated:(1)PRL increases PaI-I mRNA production in cultured granulosa cells.Inclusion of follicle stimulating hormon (FSH) with PRL has a synergistic effect on the increase of PAI-I mRNA levels.After 48 h culture in the presence of FSH with PRL,a 7.8-fold increase in PAI-I mRNA levels is observed as compared with PRL alone.The synergistic increase in PAI-I MRNA levels occurs in a dose-and time-dependent manner;(2)the increase in PAI-I mRNA synthesis in the cells by PRL alone,or PRL in combination with FSH,is well correlated with the changes of PAI-I activity levels in the conditioned media.

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引用本文：

刘以训, 柳海珍, 陈毅军, TorNY. 促乳素抑制培养的大鼠颗粒细胞中卵泡素介导的组织纤溶酶原激活因子的表达[J]. 生理学报 1998; 50 (1): .

Li Wenjue. . Acta Physiol Sin 1998; 50 (1): (in Chinese with English abstract).