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大鼠脊髓内甲啡肽、强啡肽A-(1—13)和吗啡在镇痛中的相互加强作用

黄龙, 任民峰, 吕俊华, 韩济生

中国医学科学院基础医学研究所药理研究室；北京医科大学生理教研室；大连医学院生理教研室

摘要

1.大景以辐射热甩尾法测痛。应用脊髓蛛网膜下腔,连续累加注射法观察药物的镇痛作用。脊髓蛛网膜下腔单独注射甲啡肽100#mu#mol不能提高痛阈,但与吗啡或强啡肽A-(1-13)合用时,可明显加强后者的镇痛作用。2.脊髓蛛网膜下腔单独注射强啡肽A-(-13)2.5#mu#mol本身无镇痛作用,但与吗啡合用时,却能明显加强吗啡的镇痛作用。3.该文对连续累加注射法的特点及三类阿片受体及其配基对痛觉的调制作用,进行了讨论。

关键词： 吗啡; 甲啡肽; 强啡肽A-(1-13); 脊髓蛛网膜下腔注射; 镇痛

Mutual potentiation of the analgesic effects of [met~(5)] enkephalin,dynorphia A-(1-13) and morphine in the spinal cord of the rat

Huang Long, Ren Mingfeng, Lu Junhua, Han Jisheng

Department of Physiology, Beijing Medical University. Beijing, China；Department of Phyarmacology, Institute of Basic Medical Science, Chinese Academy of Medical Sciences

Abstract

1.Nociception of the rat was assessed by measuring the tail flick latency (TFL)
with thermal irradiation. Drugs were administered intrathecally in serially increasing doses to observe their analgesic effect. Intrathecal injection of 100 #mu#mol of [met~(5)] enkephalin(MEK)alone produced no significant increase in TFL. However, when the subthreshold dose of MEK was administered in combination with morphine or dynorphin A-(1-13), a marked potentiation of the analgesic effect of the latter two was observed. 2.While 2.5 #mu#mol of dynorphin A-(1-13) alone produced no significant increase in TFL after its intrathecal injection, it potentiated the analgesic effect produced by serially increasing doses of morphine. 3. The methodology of serial intrathecal injections of increasing doses of drugs, as well the mutual influence of ligands specific for 3 types of opioid receptors in pain modulation are discussed.

Key words: Morphine;[met~(5)] enkephalin;Dynorphia A-(1-13);Intrathecal Injeceion;Analgeata

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引用本文：

黄龙, 任民峰, 吕俊华, 韩济生. 大鼠脊髓内甲啡肽、强啡肽A-(1—13)和吗啡在镇痛中的相互加强作用[J]. 生理学报 1987; 39 (5): .

Huang Long, Ren Mingfeng, Lu Junhua, Han Jisheng. Mutual potentiation of the analgesic effects of [met~(5)] enkephalin,dynorphia A-(1-13) and morphine in the spinal cord of the rat. Acta Physiol Sin 1987; 39 (5): (in Chinese with English abstract).