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侧脑室内注射微量五肽胃泌素对大鼠胃肠推进运动的影响

李希成, 张长城, 潘喜娟, 刘盛田, 周宗惠

第三军医大学生理教研室. 重庆；遵义医学院生理教研室. 贵州

摘要

以炭粉混悬液在大鼠小肠内推进距离占小肠全长的百分数,作为胃肠推进运动的指标,观察了侧脑室内注射微量五肽胃泌素(G_(5))对这一推进运动的影响。结果如下:(1)侧脑室内(ICV)注射c_(5)(1μg/10μl、2μg/10μl 或4μg/10μl)可显著增强胃肠推进运动(P<0.01—0.001)。切断两侧膈下迷走神经、阿托品皮下(0.2mg/kg)或侧脑室内(5μg/10μl)注射均可完全阻断这一增强效应(P<0.001)。(2)肌注较大剂量G_(5)(20μg/100g)可显著增强胃肠推进运动(P<0.001),这一效应亦可为皮下注射阿托品(0.2mg/kg)所完全阻断(P<0.001)。

关键词： 五肽胃泌素; 侧脑室; 微量注射; 胃肠推进运动; 迷走神经; 阿托品

Effect of intracerebro-ventricular microinjection of pentagastrin on gastrointestinal propulsive motility in the rat

Li Xicheng, Zhang Changcheng, Pan Xijuan, Liu Shengtian, Zhou Zonghui

Department of physiology, Third Medical College of PLA. Chongqing, China；Department of Physiology, Zunyi Medical College. Quizhou, China

Abstract

The purpose of this study is to observe the effect of intracerebroventricular (ICV) microinjection of pentagastrin(吼)on gastrointestinal propulsive . motility in the rat. The distance travelled by the charcol meal through the small intestine measured in terms of percentage of its total length was taken as the index of gastrointestinal propulsive motility. The results were as follows: (1) Injection of G_(5)(1 #mu#g/10 #mu#l, 2 #mu#g/10 #mu#l or 4 #mu#g/10 #mu#l) into the cerebro-ventricle significantly strengthened the gastrointestinal propulsive motility (P<0.01-0.001). These effects were abolished by vagotomy or by injection of atropine into the hypoderm (0.2 mg/kg) or into the cerebro-ventricle (5 #mu#g/10 #mu#l). (2) The gastrointestinal propulsive motility inceased markedly (P<0.001) after injection of a larger dose of G_(2) (20 #mu#g/1OOg) into the muscle. These effects were completely blocked by subcutaneous injection of atropine (0.2 mg/100g) (P<0.001). (3) No effects (P>0.05) were found in the gastrointestinal propulsive motility after intramuscular or intracerebro-ventricular injection of regitine (2.5 mg/kg im, 50 #mu#g/10 #mu#l ICV) or propranolol (5 mg/kg im, 50 #mu#g/50 #mu#l ICV). These results indicate that intracerebro-ventricular administration of G_(6) acts initially on certain cholinergic system, the enhanced activity of which is mediated by vagal nerve to cause some increase of gastrointestinal propulsive motility.

Key words: Pentagastrin (G_(5));Cerebro-ventricle;Microinjection;Gastrointestinal propulsive motility;Vagal nerve;Atropine

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引用本文：

李希成, 张长城, 潘喜娟, 刘盛田, 周宗惠. 侧脑室内注射微量五肽胃泌素对大鼠胃肠推进运动的影响[J]. 生理学报 1985; 37 (3): .

Li Xicheng, Zhang Changcheng, Pan Xijuan, Liu Shengtian, Zhou Zonghui. Effect of intracerebro-ventricular microinjection of pentagastrin on gastrointestinal propulsive motility in the rat. Acta Physiol Sin 1985; 37 (3): (in Chinese with English abstract).