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在鸡慢肌神经支配下由快肌碎片形成的新生肌肉保持快肌的某些性质

毋望远, 陆大荇, 朱德行

中国科学院上海生理研究所. 上海

摘要

该工作使用肌肉的切碎移植的方法来作快、慢肌肉的运动神经的交叉支配。将鸡右侧前背阔肌(慢)摘除而使其神经留在原位,再将左侧后背阔肌(快)取出切碎并放在右前肌的空位(第一组),在另一组动物是将右前肌摘出切碎后仍放回原位(第二组)。数月后用电刺激慢神经并用等长杠杆记录肌肉的收缩。第一组动物的新生肌肉的单收缩显然比第二组快些,而且在高频(250/s)刺激下表现抑制现象。第二组动物的新生肌肉不但收缩较慢,而且与前背阔肌一样不产生高频抑制。上述现象证明鸡的由快肌碎片形成的新生肌肉仍保持快肌的某些性质,而且在慢肌神经支配下仍能如此。

关键词： 交叉神经; 肌肉移植; 维金斯基抑制

Retention of some properties of fast muscle in the regenerated muscle grown from fast muscle slices in spite of flow motor innervation in chick

Wu Wangyuan, Lu Daxing, Zhu Dexing

Shanghai Institute of Physiology, Academia Sinica. Shangkai, China

Abstract

The determination of muscle types has been ascribed to motor innervation by many authors. In the attempt to further study the gene expression of skeletal muscle, two hypotheses have been raised, which, though different from each other, are all based on the assumption that myoblasts are bipotent and the choice of one from the two options of development is dictated by the nerve, but his assumption is not supported by the present work which, conversely, has to be interpreted otherwise at least in chick. The right anterior Latissimus Dorsi (slow) of 3-week-old chick is extirpated, the vacant place is either occupied by 1 mm slices of fast muscle (left posterior Latissimus Dorsi) in the first group of animals, or occupied by slices of slow muscle (right ALD) in the second group of animals. The muscle slices are consolidated by blood clot and left waiting for the sequential events of necrosis, regeneration and re-innervation by the remaining slow nerve to happen. After 2-17 mo (mostly 2-6 mo) the muscle contraction is recorded with isometric lever by stimulating the slow nerve. All regenerated muscles of the 15 animals of the first group and 14 animals of the second group exhibit tetanic contractions when tetanically stimulated but 12 in the first group and 8 in the second group exhibit single twitch when single shock is given. The single twitches are significantly faster in the first group, than in the second as shown in the table. Still different are the responses to high frequency shocks applied to the slow nerve that the muscles grown from minced fast (first group) all exhibit Wedensky inhibition as the normal PLD do, while muscles of the other group exhibit no inhibition even at 460/s as the normal ALD do. Wedensky inhibition has been claimed to be due to changes on the post-synaptic membrane rather than changes on the nerve terminal. The above result again proves its irrelevance to nerve and that the retention of some properties of the fast muscle in the newlv rezenerated muscle is verv nrobablv through satellite cells (presumptive myowast in muscle) in spite of incompatible innervation. H previous work with histochemical observations by the present authors leads to the same conclusion that the commitment of a muscle to be fast or slow does not necessarily take place after innervation. Since the tetanic contractions of the muscles of the two groups are all like that of the ALD, the exogenous neural factor seems to be reasonably involved.

Key words: Cross-flnnervation;Muscle transplantation;Wedensky inhibition

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引用本文：

毋望远, 陆大荇, 朱德行. 在鸡慢肌神经支配下由快肌碎片形成的新生肌肉保持快肌的某些性质[J]. 生理学报 1985; 37 (1): .

Wu Wangyuan, Lu Daxing, Zhu Dexing. Retention of some properties of fast muscle in the regenerated muscle grown from fast muscle slices in spite of flow motor innervation in chick. Acta Physiol Sin 1985; 37 (1): (in Chinese with English abstract).