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肾动脉内注射辣椒素兴奋肾神经传入纤维的自发活动

马慧娟, 武宇明, 马会杰, 张丽华, 何瑞荣

河北医科大学基础医学研究所生理室. 石家庄 050017

摘要

应用记录肾传入神经多单位和单位放电的方法,观察肾动脉内注射辣椒素对麻醉家兔肾神经传入纤维自发放电活动的影响.结果表明:(1)肾动脉内注射辣椒素20、40和60nmol/kg可呈剂量依赖性地兴奋肾传入纤维的活动,而动脉血压不变;(2)静脉内预先应用辣椒素受体阻断剂钌红(40 mmok/kg),可完全阻断辣椒素对肾传入纤维的兴奋作用.(3)静脉内预先注射一氧化氮合酶抑制剂L-NAME(0.1 mmok/kg),能延长并增强肾传入神经对辣椒素的反应.以上结果提示:肾动脉内应用辣椒素可兴奋肾传入纤维的自发放电活动.一氧化氮作为抑制因素参与辣椒素诱导的肾传入神经兴奋。

关键词： 辣椒素; 肾传入神经; 单位放电; 钌红; L-硝基精氨酸甲酯

Intrarenal artery injection of capsaicin activates spontaneous activity of renal afferent nerve fibers

Ma Huiiuan, Wu Yuming, Ma Huijie, Zhang Lihua, He Ruirong

Department of Physiology, Institute of Basic Medicine, Hebei Medical University. Shijiazhuang 050017,China

Abstract

The effects of intrarenal artery injection of capsaicin on multi- and single-unit spontaneous discharges of renal afferent nerve fibers were investigated in anesthetized rabbits. The results obtained are as follows: (1) intrarenal artery injection of capsaicin (20, 40, and 60 nmol/kg) increased the renal afferent nerve activity (ARNA) in a dose-dependent manner with unchanged arterial pressure; (2) pretreatment with ruthenium red (40 mmol/kg), a capsaicin receptor antagonist, completely abolished the effect of capsaicin; and (3) pretreatment with a nitric oxide synthase inhibitor L-NAME ( N6-nitro-L-arginine methylester, 0.1 mmol/kg), significantly enhanced the ARNA response to capsaicin. The results suggest that intrarenal artery injection of capsaicin can activate ARNA via capsaicin receptors in anesthetized rabbits and that nitric oxide may be involved in regulating the activity of renal sensory nerve fibers as an inhibitory neurotransmitter.

Key words: Capsaicin;Renal afferent nerve;Unit-activity;Ruthenium red ;L-NAME

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引用本文：

马慧娟, 武宇明, 马会杰, 张丽华, 何瑞荣. 肾动脉内注射辣椒素兴奋肾神经传入纤维的自发活动[J]. 生理学报 2003; 55 (5): .

Ma Huiiuan, Wu Yuming, Ma Huijie, Zhang Lihua, He Ruirong. Intrarenal artery injection of capsaicin activates spontaneous activity of renal afferent nerve fibers . Acta Physiol Sin 2003; 55 (5): (in Chinese with English abstract).