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胞内钙与蛋白激酶C对人脐静脉内皮细胞组织因子途径抑制物及组织因子表达的影响

汉建忠, 李俊成, 瞿树林, 贺石林

湖南医科大学生理教研室. 湖南, 长沙 410078

摘要

为探讨细胞内钙及蛋白激酶C在血管内皮细胞释放组织因子(TF)，组织因子途径抑制物(TFPI)中的作用，该文采用钙离子载入剂A_(23187)与蛋白激酶C激动剂佛波脂(PMA)刺激原代培养人脐静脉内皮细胞(HUVEC)，观察各实验组内皮细胞冻融液中TF活性及条件培养液中TFPI活性的变化，以一期凝固法测TF活性，二期生色法测定TFPI活性。结果显示：A_(23187)，PMA及A_(23187)+PMA组TF活性较对照明显升高(P＜0.001)，三组中A_(23187)和A_(23187)+PMA组活性明显低于PMA组(P＜0.05)，但前两组间无显著性差异(P＞0.05)；A_(23187)组TFPI活性与对照无明显差别，但PMA与A_(23187)+PMA组TFPI活性较对照及A_(23187)组明显升高(P＜0.01)。结果提示：细胞内钙升高及PKC激活促进血管内皮细胞TF的合成，以PKC为强；PKC促进血管内皮细胞释放TFPI，而钙对此无明显作用。

关键词： 血管内皮细胞; 组织因子; 组织因子途径抑制物; 蛋白激酶C; 钙

Roles of intercellar Ca~(2+) and protein kinase C played in tissue factor pathway inhibitor and tissue factor expression in human umbilic vein endothelial cells

Han Jianzhong, Li Juncheng, Qu Shulin, He Shilin

Department of Physiology, Hunan Medical University. Changsha 410078, Hunan, China

Abstract

The purpose of the present study was to elucidate the roles that protein kinase C (PKC) and calcium played in the tissue factor (TF) synthesis and tissue factor pathway inhibitory (TFPI) release in human umbilic vein endothelial cells (HUVEC). A_(23187) was used to represent calcium ionophore and phorbol 12-myristate 13-acetate (PMA) as that of PKC activator. TF activity in the lysed HUVEC was measured using one stage clotting assay. TFPI activity in the conditioned medium of HUVEC was assessed by the two-step chromoogenic method. The results showed that the TF activities in A_(23187), PMA and A_(23187)+PMA groups were remarkably higher (P<0.01) than that in control. Among the three treated groups, the TF activities in both A_(23187) group and A_(23187)+PMA group were lower than that in the PMA group (P<0.05), but the difference between the former two groups was statically insignificant (P>0.05).

Key words: Vascular endothelial cell;Tissue factor;Tissue factor pathway inhibitor;Protein kinase C;Calcium

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引用本文：

汉建忠, 李俊成, 瞿树林, 贺石林. 胞内钙与蛋白激酶C对人脐静脉内皮细胞组织因子途径抑制物及组织因子表达的影响[J]. 生理学报 1997; 49 (6): .

Han Jianzhong, Li Juncheng, Qu Shulin, He Shilin. Roles of intercellar Ca~(2+) and protein kinase C played in tissue factor pathway inhibitor and tissue factor expression in human umbilic vein endothelial cells. Acta Physiol Sin 1997; 49 (6): (in Chinese with English abstract).