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巢蛋白mRNA在小鼠中枢神经系统发育过程中的表达

杨靖, 边玮, 景乃禾

中国科学院上海生物化学研究所. 上海 200031

摘要

巢蛋白(Nestin)属于中等纤维基因家族，在增殖较快的神经前体细胞中表达。该基因被克隆后，作为神经前体细胞的标记基因得到广泛应用。该文中，作者根据小鼠巢蛋白cDNA序列，设计了一对引物，在确定了反转录PCR反应的最佳反应条件后，详细地考察小鼠巢蛋白mRNA在中枢神经系统发育过程中的表达规律，发现巢蛋白mRNA在小鼠第10天胚胎的脑中表达，到第14天表达量达到最高，后下降；在出生后的小脑中，表达也有类似规律，出生后第5天，表达量最高，其后下降。在成年小鼠各组织中，均未检测到巢蛋白mRNA的表达。

关键词： 巢蛋白基因; 反转录PCR; mRNA表达; 神经发育

Nestin mRNA expression during the development on mouse central nervous system

Yang Jing, Bian Wei, Jing Naihe

Shanghai Institute of Biochemistry, Chinese Academy of Science. Shanghai 200031 China

Abstract

Intermediate filament Nestin is a marker of neural precursor cells. Its expression pattern during mouse brain development was studied in the present investigation. A pair of primers based on the sequence of mouse Nestin were found best to be suit for the reverse transcriptional PCR (RT-PCR) with this pair of Nestin Primer. Using RT-PCR, Nestin transcripts were detected during the development of the central nervous system (CNS) of mouse, from embryonic day 10 to adult brain. We found that the Nestin showed a transient expression in the development of cerebrum. At the embryonic day 14, the expression of Nestin mRNA reached the highest peak, and was then down-regulated. In the postnatal cerebellum, Nestin gene was also expressed in a transient pattern. The highest peak of Nestin mRNA expression emerged at postnatal day 5, and was then down-regulated. Nestin mRNA expression in 10 kinds of adult mouse tissues did not yield any positive results.

Key words: Nestin gene;Reverse transcriptional PCR;mRNA expression;Neural development

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引用本文：

杨靖, 边玮, 景乃禾. 巢蛋白mRNA在小鼠中枢神经系统发育过程中的表达 [J]. 生理学报 1997; 49 (6): .

Yang Jing, Bian Wei, Jing Naihe. Nestin mRNA expression during the development on mouse central nervous system. Acta Physiol Sin 1997; 49 (6): (in Chinese with English abstract).