过刊浏览

硝基左旋精氨酸对睡眠抑制作用的机制研究

章茜, 王书春, 乔鹏, 胡群圆, 陈力超, 王雨若

河南医科大学生理教研究室. 河南, 郑州 450052；河南职工医学院组织胚胎教研室. 河南, 郑州 450003

摘要

该文观察了硝基左旋精氨酸(L-NNA，50mg/kg，ip)和L-精氨酸(L-arg，110mg/kg，ip)对慢性植入电极的大鼠睡眠-觉醒周期的影响及中缝核5-羟色胺(5-HT)神经元免疫阳性反应的变化。结果表明：L-NNA显著抑制慢波睡眠和快眼动睡眠，使平均动脉压(MAP)升高。L-arg则使MAP显著降低，对睡眠无明显影响。预先给予L-arg可逆转L-NNA的效应。腹腔给予L-NNA后2h，中缝背核(DR)和中央上核(MnR)5-HT神经元免疫阳性细胞明显增多，与对照比较，P＜0.01，此效应可被预先给予L-arg所减弱。提示L-arg：NO通路参与睡-醒周期节律的调节。中缝核头部5-HT神经元免疫阳性反应增强可能与L-NNA抑眠作用有关。

关键词： 睡眠-觉醒周期; 一氧化氮; 中缝核; 5-羟色胺

A study of the mechanism of L-nna on sleep inhibition

Zhang Qian, Wang Shuchun, Qiao Peng, Hu Qunyuan, Chen Lichao, Wang Yuruo

Department of Physiology, Henan Medical University. Zhengzhou 450052, Henan, China

Abstract

In the present study, the sleep-waking cycle and the immunoreactivity of 5-HTcontainingneurons in rats receiving intraperitoneal injections of N-Nitro-L-arginine (L-NNA, 50 mg/kg) and L-arginine (L-arg, 110 mg/kg) were observed respectively.The results showed that injection of L-NNA suppressed both slow wave sleep (SWS) and rapid eye movement sleep (REMS) and increased mean arterial pressure (MAP). L-arg markedly attenuated MAP, whereas SWS and REMS were unchanged. The effects of L-NNA on sleep and MAP were reversed by preinjection of L-arg. In comparison with control group, the positive cells in dorsal raphe nucleus (DR) and raphe magus nucleus (MnR) increased 2 h after injection of L-NNA. The effect was attenuated by preadministration of L-arg. All these results suggest that L-arg: NO pathway is involved in theregulation of sleep-waking cycle and the increment of the positive immunoreactivity of the 5-HT-containing neurons in the DR may be related to the sleep-suppressive effects induced by L-NNA.

Key words: Sleep-waking cycle;Nitric oxide;Dorsal raphe nucleus;5-HT

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

章茜, 王书春, 乔鹏, 胡群圆, 陈力超, 王雨若. 硝基左旋精氨酸对睡眠抑制作用的机制研究[J]. 生理学报 1997; 49 (5): .

Zhang Qian, Wang Shuchun, Qiao Peng, Hu Qunyuan, Chen Lichao, Wang Yuruo. A study of the mechanism of L-nna on sleep inhibition. Acta Physiol Sin 1997; 49 (5): (in Chinese with English abstract).