过刊浏览

新生大鼠脊髓交感节前神经元的单胺能受体

蔡圣荣, 王烈成, 孔德虎, 黄振信, 马如纯

安徽医科大学生理学教研室. 安徽, 合肥 230032；安徽中医学院生理学教研室. 安徽, 合肥 230038

摘要

应用新生大鼠离体脊髓薄片的细胞内记录，观察了5-羟色胺(5-HT)、去甲肾上腺素(NA)及肾上腺素(AD)对交感节前神经元(SPN)膜电位的作用，以期阐明三种单胶受体是共存于同一细胞，还是分别存在于不同细胞？实验结果显示：(1)5－HT，NA及AD分别使57.1％(16/28)，60％(1/25)及52.4％(1/2)的SPN出现了去极化反应；(2)根据SPN对三种单肢的反应，可将SPN分成几种亚型：对三种单胺都敏感的SPN(3/19，158％)，对两种单胺都敏感的SPN(9/19，47.4％)，仅对一种单胺敏感的SPN(4/19，21.1％)以及对三种单胺都不敏感的SPN(3/19，15.8％)。结果提示三种单胺受体可三者或两者共存于同一细胞，也可分别单独存在于一个细胞。

关键词： 5-羟色胺; 去甲肾上腺素; 肾上腺素; 单胺; 交感节前神经元

Receptors of monoamine in sympathetic preganglionic neurons of neonatal rat spinal cord in vitro

Cai Shengrong, Wang Liecheng, Kong Dehu, Huang Zhenxin, Ma Ruchun

Department of Physiology, Anhui Medical University. Hefei 230032, Anhui, China；Department of Physioloy, Anhui Chinese Traditional Medical College. Hefei 230038, Anhui, China

Abstract

By means of intracellular recordings from spinal cord slices of neonatal rats in vitro,the effects of 5-hydroxytryptamine (5-HT), nor-adrenaline (NA) and adrenaline (AD)on membrane potential in sympathetic preganglionic neurons (SPN) were observed,in order to clarify whether these neuron contain a single type of the monoamine receptor or in cornbination with more than one type of receptors. The results showed that: (1) 5-HT,NA and AD induced membrane depolarization respectively in 57.11% (16/28), 60%(15/25) and 52.4% (11/21) of SPN. (2) According to the reactions of SPN to the three monoamines, several subtypes of SPN could be divided: those sensitive to all the three monoarnines (3/19), those sensitive to two of them (9/19), those only sensitive to one trpe of monoandnes (4/19) and those insensitive at all (3/19). The significance of coexistence of more than one type of the three monoamines in a single neuron remains to be elucidated.

Key words: 5-hydroxytryptamine;Noradrenaline;Adrenaline;Monoamine;Sympathetic preganglionic neuron

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

蔡圣荣, 王烈成, 孔德虎, 黄振信, 马如纯. 新生大鼠脊髓交感节前神经元的单胺能受体[J]. 生理学报 1997; 49 (5): .

Cai Shengrong, Wang Liecheng, Kong Dehu, Huang Zhenxin, Ma Ruchun. Receptors of monoamine in sympathetic preganglionic neurons of neonatal rat spinal cord in vitro. Acta Physiol Sin 1997; 49 (5): (in Chinese with English abstract).