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腺苷对缺氧时海马神经元内游离Ca~(2+)的影响

万勤, 王福庄, 要航, 刘振伟, 黄燕华, 丁爱石

军事医学科学院基础医学研究所. 北京 100850

摘要

实验用Fluo-3负载细胞，在激光扫描共聚焦显微镜下直接监测缺氧后分散培养的大鼠海马CA1区神经元内游离Ca~(2+)浓度([Ca~(2+)]_(i))的变化，观察腺苷对这种变化的影响并初步探讨其作用机制。结果发现，急性缺氧使海马神经元[Ca~(2+)]_(i)显著升高；腺苷(100μmol/L)明显抑制缺氧引起的[Ca~(2+)]_(i)增高，腺苷A1受体拮抗剂CPT以及K~(+)通道阻断剂4-AP和ATP敏感性K~(+)通道阻断glipizide均可拮抗腺苷的抑制作用。结果表明，腺苷通过其A1受体介导，可能激活4-AP和／或ATP敏感性K~(+)通道，从而抑制缺氧引起的海马神经元胞内Ca~(2+)升高。

关键词： 腺苷; 缺氧; Fluo-3; [Ca~(2+)]_(i); 海马神经元

Effect of adenosine on intracellular free calcium in cultured rat hippocampal CA1 neurons during anoxia

Wan Qin, Wang Fuzhuang, Yao Hong, Liu Zhenwei, Huang Yanhua

Institute of Basic Medical Sciences. Beijing 100850, China

Abstract

Intracellular free Ca~(2+) concentration ([Ca~(2+)]_(i)) was measured by laser scanning confocal microscope, using Ca~(2+) indicator Fluo-3 in cultured hippocampal CA1 neurons isolated from newborn rat. The results showed that acute anoxia induced a rapid increase of [Ca~(2+)], in hippocampal CA1 neurons, and this increase could be attenuated by 100 #mu#mol/L adenosine significantly. This effect of adenosine could be suppressed by adenosine Al receptor antagonist CPT or potassium channel blockers, 4-AP and glipizide.These regults suggest that adenosine activates 4-AP- or ATP-sensitive potassium channels through A1 receptors, and consequently inhibits the [Ca~(2+)]_(i), elevation in hippocampal netirons during anoxia.

Key words: Adenosine;Anokia;[Ca~(2+)]_(i);Fluo-3;Hippocampal neurons

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引用本文：

万勤, 王福庄, 要航, 刘振伟, 黄燕华, 丁爱石. 腺苷对缺氧时海马神经元内游离Ca~(2+)的影响[J]. 生理学报 1997; 49 (5): .

Wan Qin, Wang Fuzhuang, Yao Hong, Liu Zhenwei, Huang Yanhua. Effect of adenosine on intracellular free calcium in cultured rat hippocampal CA1 neurons during anoxia. Acta Physiol Sin 1997; 49 (5): (in Chinese with English abstract).