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蛋白水解酶抑制剂对人精子穿透去透明带田鼠卵子的抑制作用

张燕林, 吕容真, 王小刚, 王守真, 朱洪, 周元聪

上海实验动物研究中心. 上海；中国科学院上海生物化学研究所. 上海

摘要

该文研究了慈姑和天花粉两种天然的蛋白水解酶抑制剂对人精子穿透去透明带田鼠卵子的影响。结果显示:(1)在人精子获能期1.0×10~(-3)mol/L天花粉抑制剂可使人精子的穿卵率明显下降(P<0.01),而同样浓度的慈姑抑制剂,作用不明显。(2)去透明带田鼠卵子预先用抑制剂处理0.5h,被获能精子的穿卵率和未用抑制剂处理的卵子相似。(3)在获能后的人精子和去透明带田鼠卵子孵育期,两种抑制剂的浓度为0.5或1.0×10~(-3)mol/L时,精子的穿卵率明显下降。

关键词： 慈姑蛋白酶抑制剂; 天花粉蛋白酶抑制剂; 去透明带田鼠卵子

Inhibition of human sperm penetration into zona-free hamster oocytes by porteinase inhibitors

Zhang Yanlin, Lu Rongzhen, Wang Xiaogang, Wang Shouzhen, Zhu Hong, Zhou Yuancong

Shanghai Institute of Planned Parenthood Research. Shanghai, China；Shanghai Institute of Biochemistry, Academy Sinica

Abstract

Effects of two natural proteinase inhibitors (Trichosanthes inhibitor and Arrowhead inhibitor) on human sperm penetration into zona-free hamster oocytes
were investigated. The results showed: (1) 1.0×10~(-3) mol/L Trichosanthes inhibitor added to the spermatozoa containing medium for a period of preincubation intended to induce capacitation caused a large decrease in the spermatozoa penetration rate (P<0.01), while Arrowhead inhibitor of similar concentration did not,how significant effect. (2) The penetration rate of capacitation spermatozoa into zona-free oocytes pretreated with either inhibitor for 0.5 h was similar to that of the untreated control. (3) When either inhibitor [1.0×10~(-3) mol/L or 0.5×10~(-3) mol/L] was present in the capacitation sperm in egg incubation medium, the spermatozoa penetration rate was significantly decreased (P<0.01). (4) When 1.0×10~(-3) mol/L of either inhibitor was present during both the preincubation for capitation and the sperm/egg incubation periods, the ability of spermatozoa to penetrate zona-free oocytes was significantly decreased (P<0.01). 1.0×10~(-4) mol/L inhibitors did not show up significant effect. (5) The motility of the sperm was not affected
by the inhibitors. Thus it is the enzyme system involved in the penetration of
sperm into oocyte affected by the proteinase inhibitors.

Key words: Trichosanthes inhibitor;Arrowhead inhibitor;Zona-free hamster oocytes

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引用本文：

张燕林, 吕容真, 王小刚, 王守真, 朱洪, 周元聪. 蛋白水解酶抑制剂对人精子穿透去透明带田鼠卵子的抑制作用[J]. 生理学报 1987; 39 (4): .

Zhang Yanlin, Lu Rongzhen, Wang Xiaogang, Wang Shouzhen, Zhu Hong, Zhou Yuancong. Inhibition of human sperm penetration into zona-free hamster oocytes by porteinase inhibitors. Acta Physiol Sin 1987; 39 (4): (in Chinese with English abstract).