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蛙离体单根有髓鞘神经纤维静息电位与动作电位细胞内记录方法

周泰生

中国科学院上海生理研究所. 上海

摘要

该文介绍一种记录蛙离体单根有髓鞘神经纤维的静息和动作电位的细胞内记录方法,包括神经干标本的制作和固定、微电极和刺激电极的制备、简易防震和静息与动作电位的记录.该方法记录的静息电位和动作电位幅值,在30min 内可保持相对稳定。

关键词： 有髓鞘神经纤维; 细胞内记录; 静息电位; 动作电位

A method for intracellular recording of resting and action potential from the isolated frog single myelinated nerve flber

Zhou Taisheng

Shanghai Institute of Physiology, Academy Sinica

Abstract

A method of intracellular recording of resting potential and action potential
from the frog single mychnated nerve fiber was described, which consists of the manufacture of microelectrode, the preparation of speciman, the method of nerve stimulation and the device for reducing vibration. The amplitude of the resting and action potential could be relatively stable over a period of 30 min utes.

Key words: Myelinated nerve fibers;Intracellular recording;Resing potential;Action potential

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引用本文：

周泰生. 蛙离体单根有髓鞘神经纤维静息电位与动作电位细胞内记录方法[J]. 生理学报 1987; 39 (2): .

Zhou Taisheng. A method for intracellular recording of resting and action potential from the isolated frog single myelinated nerve flber. Acta Physiol Sin 1987; 39 (2): (in Chinese with English abstract).