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中国东方铃蟾(Bombina orientalis)皮肤中C_(-12-h)肽的分离与鉴定

陈枫, 赵轶千

同济医科大学生理教研室

摘要

东方铃蟾鲜皮的甲醇提取液,可引起大鼠离体平滑肌的收缩。此液经碱性氧化铝柱层析分离,其80%乙醇洗脱的 C 组分显示生物活性。C 组分再经葡聚糖凝胶 G-15柱分离,其活性较强的早期洗脱组分 C_(_12),可被糜蛋白酶水解灭活,但其活性并不被5-HT 拮抗剂赛庚啶[2×10~(-6)mol/L]完全拮抗。继用反相高效液相色谱(RP-HPLC)分析,见分离出的 C_(_12_h)峰与铃蟾肽~(*)(Bombesin,BBS)的出峰时间相同,且具相同的氨基酸组成。上述实验结果提示,C_(_12_h)肽可能就是铃蟾肽。

关键词： 东方铃蟾; C_(12_h)肽; 铃蟾肽

Isolation and identification of C_(12-h) peptide from the skin of Bombina Orientalis in China

Chen Feng, Zhao Yiqian

Department of physiology, Tong-Ji Medical University

Abstract

caused contraction of the smooth muscles of rat in vitro. The crude extracts were isolated by column chromatography of alkaline alumina, and a bioactive fraction was obtained from the 80%ethanol eluate. Submitting the fraction on Sephadex G_(-12) column.we found that the early eluate C_(-12),showing more bioactivity, was inactivated by hydrolysis with chymotrypsin, but not completely antagonized by cyproheptadine. C_(-12) was further analyzed by reversed phase-high performance liquid chromatography (RP-HPLC).The elution time of C_(-12-h) peak was the same as that of bombesin, and these two peptides had the same amino acid composition. These experimental results suggest that C_(-12-h) peptide is most probably bombesin.

Key words: Bombina orientalis;C_(-12-h) peptide;Bombesin

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引用本文：

陈枫, 赵轶千. 中国东方铃蟾(Bombina orientalis)皮肤中C_(-12-h)肽的分离与鉴定[J]. 生理学报 1987; 39 (2): .

Chen Feng, Zhao Yiqian. Isolation and identification of C_(12-h) peptide from the skin of Bombina Orientalis in China. Acta Physiol Sin 1987; 39 (2): (in Chinese with English abstract).