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促性腺激素释放激素及多巴胺对斜带石斑鱼生长激素分泌及其mRNA表达的调控

冉雪琴, 李文笙, 林浩然

中山大学水生经济动物研究所及广东省重点实验室. 广东, 广州 510275

摘要

研究斜带石斑鱼生长激素分泌及其mRNA表达的调控规律对于性别分化的控制、临床药物的选择,以及石斑鱼的增养殖等均具有重要的理论意义和实践意义.本文应用静态孵育系统,采用放射免疫测定法和化学发光液相杂交实验,研究GnRH和DA对斜带石斑鱼GH分泌、GH mRNA合成的调控作用.100 nmol/L sGnRH作用斜带石斑鱼脑垂体碎片1～24 h,明显促进GH的释放和GH mRNA的合成,并具有时间依存性。

关键词： 生长激素; 生长激素mRNA; 静态孵育; 促性腺激素释放激素; 多巴胺; 阿扑吗啡; 斜带石斑鱼 (Epinephelus coioides)

Stimulatory effects of gonadotropin-releasing hormone and dopamine on growth hormone release and growth hormone mRNA expression in Epinephelus coioides

Ran Xueqin, Li Wensheng, Lin Haoran

Institute of Aquatic Economic Aninals and Guangdong Provincial Key Laboratory for Aquatic Economic Animals, Zhonghsan University. Guangzhou 510275, China；School of Animal Science, Guizhou University. Guiyang 550025, China

Abstract

Gonadotropin-releasing hormone (GnRH) and dopamine (DA) can stimulate growth hormone (GH) release, but their effects on GH mRNA synthesis are controversial and deficient in fish. Orange-spotted grouper (Epinephelus coioides) is a hermaphroditic marine fish with sex reversal. Few data are available concerning the regulation of GH in grouper. In the present study, the effects of GnRH and DA on GH release and GH mRNA expression were determined using pituitary fragments of orange-spotted grouper under static culture conditions. After incubation from 1 h to 24 h, salmon GnRH (sGnRH, 100 nmol/L) stimulated the release of GH and increased the level of GH mRNA time-dependently.

Key words: Growth hormone (GH);GH mRNA;Static incubation;Gonadotropin-releasing hormone (GnRH);Dopamine (DA);Apomorphine(APO);

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引用本文：

冉雪琴, 李文笙, 林浩然. 促性腺激素释放激素及多巴胺对斜带石斑鱼生长激素分泌及其mRNA表达的调控[J]. 生理学报 2004; 56 (5): .

Ran Xueqin, Li Wensheng, Lin Haoran. Stimulatory effects of gonadotropin-releasing hormone and dopamine on growth hormone release and growth hormone mRNA expression in Epinephelus coioides. Acta Physiol Sin 2004; 56 (5): (in Chinese with English abstract).