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慢性间歇性低氧降低急性缺氧对大鼠肺动脉平滑肌细胞膜上电压门控性钾通道的抑制

唐碧, 唐明, 杜以梅, 刘长金, 洪志刚, 骆红艳, 胡新武, 宋元龙, 席姣娅, Jurgen Hescheler

华中科技大学同济医学院生理系. 湖北, 武汉 430030；Institute of Neurophysiology, University of Cologne, D-50931 Cologne Germany

摘要

为了从离子通道水平上探讨机体低氧适应的离子机制,本实验将雄性SD大鼠随机分为常氧对照组和慢性间歇性低氧组[氧浓度(10±0.5)%,间断缺氧每天8 h].用酶解法急性分离单个大鼠肺内动脉平滑肌细胞(pulmonary artery smoothmuscle cells,PASMCs),以全细胞膜片钳技术记录PASMCs膜上的电压门控性钾通道(voltage-gated potassium channel,Kv)电流,观察急性缺氧对慢性间歇性低氧大鼠PASMCs的KV的影响,为机体适应低氧能力提供实验依据。

关键词： 肺动脉; 电压门控性钾通道; 低氧; 膜片钳

Chronic intermittent hypoxia decreases acute hypoxic inhibition of voltage-gated potassium channel in rat pulmonary arterial smooth muscle cells

Tang Bi, Tang Ming, Du Yimei, Liu Cnangjin, Hong Zhigang, Luo Hongyan, Hu Xinwu, Song Yuanlong, Xi Jiaoya, Jurgen Hescheler

Department of Physiology, Tongji Medical College of Huazhong University of Science and Technology. Wuhan 430030, China；Department of Geriatrics, the Union Hospital, Tongji Medical College, Huazhong University of Science and Technology. Wuhan 430022, China；Institute of Neurophysiology, University of Cologne, D-50931 Cologne Germany

Abstract

For determination the ionic mechanisms of the hypoxic acclimatization at the level of channels, male Spradue-Dawley rats were divided into two groups control normoxic group and chronic intrmittent hypoxic group [O_(2) concebtratuib: (10±0.5)%, hypoxia 8h a day]. Using whole cell patch-clamp technique, voltage-gated potassium channel currents [(IK_(v)] wre recorded in freshly isolated pu;monary arterial smooth muscle cells (PASMCs) of rat with acute isolated method.

Key words: Pulmonary artery;Voltage Gated potassium channel;Patch clamp;Hypoxia

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引用本文：

唐碧, 唐明, 杜以梅, 刘长金, 洪志刚, 骆红艳, 胡新武, 宋元龙, 席姣娅, Jurgen Hescheler. 慢性间歇性低氧降低急性缺氧对大鼠肺动脉平滑肌细胞膜上电压门控性钾通道的抑制[J]. 生理学报 2004; 56 (5): .

Tang Bi, Tang Ming, Du Yimei, Liu Cnangjin, Hong Zhigang, Luo Hongyan, Hu Xinwu, Song Yuanlong, Xi Jiaoya, Jurgen Hescheler. Chronic intermittent hypoxia decreases acute hypoxic inhibition of voltage-gated potassium channel in rat pulmonary arterial smooth muscle cells. Acta Physiol Sin 2004; 56 (5): (in Chinese with English abstract).