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高频电刺激股神经减小大鼠心肌缺血-再灌注后的梗塞范围

董京辉, 刘宜先, 赵娟, 马会杰, 郭淑梅, 何瑞荣

河北医科大学基础医学研究所生理研究室. 河北, 石家庄 050017

摘要

通过氨基甲酸乙酯麻醉大鼠观察股神经电刺激对缺血-再灌注心肌的影响,旨在证实外周神经刺激对心肌有无保护效应,并明确其可能的作用机制.心肌缺血区和梗塞区分别用伊文思蓝和氯化硝基四氮唑蓝染色确定,心肌梗塞范围定义为心肌梗塞区重量占心肌缺血区重量的百分比.所得结果如下:(1)在心肌缺血30 min和再灌注120 min过程中,梗塞心肌占缺血心肌的(54.96+0.82)%。

关键词： 缺血-再灌注; 电刺激; 阿片肽; 纳洛酮; 格列苯脲

High-frequency electrical stimulation of femoral nerve reduces infarct size following myocardial ischemia-reperfusion in rats

Dong Jinghui, Liu Yixian, Zhao Juan, Ma Huijie, Guo Shumei, He Ruirong

Department of Physiology, Institute of Basic Medicine, Hebei Medical University. Shijiazhuang 050017, China

Abstract

The effects of femoral nerve electrostimulation (FNES) on ischemia-reperfused myocardium were examined in the urethaneanesthetized rats to determine whether FNES may provide cardioprotection and to observe the possible mechanism. The area at risk (AR) and infarct area (IA) were determined using Evans blue and nitro-blue tetrazolium staining, respectively. Infarct size (IS) was defined as 100 × IA/AR (%). The results are as follows: (1) During 30 min myocardial ischemia and subsequent 120 min reperfusion,the myocardial infarct size occupied (54.96±0.82)% of the area at risk.

Key words: Ischemia-reperfusion;Electrostimulation;Opioid;Naloxone;Glibenclamide

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引用本文：

董京辉, 刘宜先, 赵娟, 马会杰, 郭淑梅, 何瑞荣. 高频电刺激股神经减小大鼠心肌缺血-再灌注后的梗塞范围[J]. 生理学报 2004; 56 (5): .

Dong Jinghui, Liu Yixian, Zhao Juan, Ma Huijie, Guo Shumei, He Ruirong. High-frequency electrical stimulation of femoral nerve reduces infarct size following myocardial ischemia-reperfusion in rats. Acta Physiol Sin 2004; 56 (5): (in Chinese with English abstract).