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慢性复合应激增强大鼠空间学习和记忆能力

刘能保, 李辉, 刘向前, 孙臣友, 陈少容, 张敏海, 刘少纯, 汪薇曦

华中科技大学同济医学院解剖学系组织胚胎学教研室. 湖北, 武汉 430030

摘要

该文观察了慢性复合应激对大鼠学习与记忆功能的影响.实验采用成年Wistar大鼠,将其随机分成应激组和对照组.采用垂直旋转、睡眠剥夺、噪音刺激和夜间光照4种应激原,无规律地交替刺激动物6周,每天6 h,制作慢性复合应激动物模型.采用Morris水迷宫和Y-迷宫测试大鼠学习与记忆成绩,并用Cresyl violet染色法对大鼠海马结构进行神经细胞计数。

关键词： 慢性复合应激; 学习与记忆; Morris 水迷宫; Y-迷宫; 海马

Chronic multiple stress enhances learning and memory capability in rats

Liu Nengbao, Li Hui, Liu Xiangqian, Sun Chenyou, Cheng Shaorong, Zhang Minhai, Liu Shaochun, Wang Weixi

Department of Histology and Embryology of Anatomy, Tongji Medical School of Hua Zhong University of Science and Technology. Wuhan 430030, China

Abstract

The present study aimed at investigating the effects of chronic multiple stress on learning and memory functions of rats. Adult male Wistar rats were randomly divided into stressed and control groups. Rats in the stressed group were irregularly and alternately exposed to the situation of vertical revolution, sleep deprivation, noise stimulation, and night illumination 6 h per day for 6 weeks to prepare a chronic multiple stressed model. Learning and memory performance of rats was measured by using Morris water maze first and Y-maze afterwards. Neurons in the dentate gyrus(DG), CA3 and CA1 regions of the hippocampus were stained by using Cresyl violet method and counted. The results showed that: (1) After chronic multiple stress, compared with the control rats, the escape latency to the hidden platform in Morris water maze was significantly shortened in stressed rats.

Key words: Chronic multiple stress;Learning and memory;Morris water maze;Y-maze hippocampus;

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引用本文：

刘能保, 李辉, 刘向前, 孙臣友, 陈少容, 张敏海, 刘少纯, 汪薇曦. 慢性复合应激增强大鼠空间学习和记忆能力[J]. 生理学报 2004; 56 (5): .

Liu Nengbao, Li Hui, Liu Xiangqian, Sun Chenyou, Cheng Shaorong, Zhang Minhai, Liu Shaochun, Wang Weixi. Chronic multiple stress enhances learning and memory capability in rats. Acta Physiol Sin 2004; 56 (5): (in Chinese with English abstract).