过刊浏览

心室内和心外膜应用腺苷对延髓PGL神经元电活动的影响

马秀英, 范振中, 何瑞荣

河北医科大学基础医学研究所生理室. 河北, 石家庄 050017

摘要

在35只切断两侧缓冲神经和迷走神经的麻醉大鼠，观察心室内注射腺苷和心外膜涂布腺苷对延髓腹外侧头端区PGL神经元自发电活动的影响。结果如下：(1)35只大鼠共记录到121个自发放电单位，平均放电频率为22.5±1.9spikes/s。(2)心室内冲击注射腺苷(0/5μmol/kg，0.1ml)时，BP先升(△1.7±0.2kPa，P＜0.001)后降(△4.6±0.5kPa，P＜0.001)，HR减慢(△126.5±12.3bpm，P＜0.001)；35个PGL神经元自发放电单位中，30个单位的放电频率由21.9±2.6增至29.2±3.4spikes/s(P＜0.001)，3个单位不变，2个单位减少。(3)心外膜涂布腺苷(20mmol/L)，动脉血压和心率的变化不明显，22个PGL神经元自发放电频率由18.8±1.9增至26.9±2.8spikes/s(P＜0.001)，3个单位的放电频率无变化。(4)静脉注射选择性腺苷A_(1)受体拮抗8－cyclopentyl-1，3－dipropylxanthine(DPCPX，500μg/kg)可完全阻断腺苷对PGL神经元的兴奋效应。(5)在左右房室沟涂布85％酚或切除双侧星状神经节后，腺苷激活PGL神经元的效应即行消失。结果提示，腺苷可通过人受体激活心交感神经传入纤维，进而兴奋PGL神经元。

关键词： 腺苷; 腺苷受体; 心交感传入纤维; 延髓腹外侧头端区

Effects of intraventricular and epicardial application of adenosine on electrical activity of medullary pgl neurons

Ma Xiuying, Fan Zhenzhong, He Ruirong

Department of Physiology, Institute of Basic Medicine, Hebei Medical University. Shijiazhuang 050017, Hebei, China

Abstract

The effects of intraventricular injection and epicardial application of adenosine on spontaneous electrical activity of nucleus paragigantocellularis lateralis (PGL) neurons in rostral ventrolateral medulla(RVLM) were examined in 35 anesthetized rats with sinoaortic denervation and vagotomy. The results obtained were as follows: (1) The sPOntaneous discharge of 121 PGL neurons (mean discharge rate: 22.5+-1.9 spikes/s) were recorded in 35 rats. (2) In resp0nse to intraventricular injection of adenosine (0.5 #mu#mol/kg), mean arterial pressure (MAP) was initially increased by 1.7+-0.2 kPa(P<0.001) and subsequently decreased by 4.6+-0.5 kPa(P<0.001), while the heart rate (HR) was decreased by 126.5+-12.3 bpm (P<0.001). Of 35 PGL spontaneous discharge units responsive to intraventricu1ar injection of adenosine, 30 showed an average increase from 21.9+-2.6 to 29.2+-3.4 spikes/s (P<0.001), 3 with no change, while 2 with a decrease.

Key words: Adenosine;Adenosine receptors;Cardiac sympathetic afferents;Rostral ventrolateral medulla

收稿日期：　　录用日期：

通讯作者：　　E-mail:

引用本文：

马秀英, 范振中, 何瑞荣. 心室内和心外膜应用腺苷对延髓PGL神经元电活动的影响 [J]. 生理学报 1997; 49 (5): .

Ma Xiuying, Fan Zhenzhong, He Ruirong. Effects of intraventricular and epicardial application of adenosine on electrical activity of medullary pgl neurons. Acta Physiol Sin 1997; 49 (5): (in Chinese with English abstract).