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急性低氧对体外培养乳鼠心肌细胞肌红蛋白的影响

柴旦, 周兆年

中国科学院上海生理研究所低氧室. 上海 200031

摘要

该实验观察了低氧、复氧时培养的乳鼠心肌细胞肌红蛋白(Mb)、cAMP、心肌收缩频率的变化以及磷酸二酯酶抑制剂茶碱和抑制肌质网上钙释放的普鲁卡因对低氧下心肌细胞Mb表达和心肌细胞收缩频率的影响。结果表明，随低氧时间的延长Mb增加，cAMP和心肌收缩下降，Mb、cAMP和心肌细胞收缩频率经复氧可以得到恢复。普鲁卡因使低氧时心肌细胞的收缩频率更慢和Mb的表达减弱；茶碱使低氧下心肌细胞的收缩频率和Mb的表达增加。实验结果提示：低氧时Mb表达增加、cAMP和心肌收缩频率下降可能是对低氧的适应性改变，而Mb表达可能由胞内钙与cAMP参与。

关键词： 低氧; 复氧; 培养心肌细胞; 肌红蛋白; 环磷酸腺苷; 心肌细胞收缩

Effects of hypoxia on myoglobin in cultured rat myocytes

Chai Dan, Zhou Zhaonian

Institute of Physiology, Chinese Academy of Sciences. Shanghai 200031, China

Abstract

The effects of hyoxia and reoxygenation on myoglobin (Mb), cAMP, myocardial contractility and the effects of theophylline (an inhibitor of phosphodiesterase), procaine (an inhibitor of SR calcium release) on Mb expression were studied in cultured myocytes under hypoxic condition. Our results showed that hypoxia increased the Mb expression,but decreased cAMP and myocardial contractility. All these effects of hypoxia were recovered upon reoxygenation. The increase of Mb expression requires the participation of intracellular calcium and cAMP.

Key words: Hypoxia;Reoxygenation;Cultued myocyte;Myoglobin;cAMP;Mycardial contractility

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引用本文：

柴旦, 周兆年. 急性低氧对体外培养乳鼠心肌细胞肌红蛋白的影响 [J]. 生理学报 1997; 49 (5): .

Chai Dan, Zhou Zhaonian. Effects of hypoxia on myoglobin in cultured rat myocytes. Acta Physiol Sin 1997; 49 (5): (in Chinese with English abstract).